Products for Research Use Only
RegisterLogin

TFF1 ELISA Kit (Rat)

CAT: 0247-OKCD02902-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKCD02902-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Trefoil Factor 1 (TFF1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Trefoil Factor 1 (TFF1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Cytochrome b-245 alpha chain
Gene Aliases
Alpha-subunit p22; cytochrome b (558) alpha chain; cytochrome b-245 light chain; cytochrome b-245, alpha polypeptide; cytochrome b558 alpha-subunit; cytochrome b558 subunit alpha; neutrophil cytochrome b 22 kDa polypeptide; p22 phagocyte B-cytochrome; p22phox; p22-phox; Phox; superoxide-generating NADPH oxidase light chain subunit.
Gene ID
79129
Accession Number
NP_001002992.1
Reactivity
Rat|Rattus norvegicus
Target
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trefoil Factor 1 (TFF1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Trefoil Factor 1 (TFF1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Trefoil Factor 1 (TFF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Trefoil Factor 1 (TFF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78.12-5000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 78-88 82 EDTA Plasma (n=5) 89-103 94 Heparin Plasma (n=5) 89-103 93 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 78-88 82 Serum (n=5) 78-88 82 EDTA Plasma (n=5) 89-103 94 EDTA Plasma (n=5) 89-103 94 Heparin Plasma (n=5) 89-103 93 Heparin Plasma (n=5) 89-103 93
Sensitivity
27 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-TFF1 Microplate 96 Wells (12 x 8 Well strips) TFF1 Lyophilized Standard 2 100X Biotinylated TFF1 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-TFF1 Microplate 96 Wells (12 x 8 Well strips) Anti-TFF1 Microplate 96 Wells (12 x 8 Well strips) TFF1 Lyophilized Standard 2 TFF1 Lyophilized Standard 2 100X Biotinylated TFF1 Detector Antibody 1 x 120 uL 100X Biotinylated TFF1 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Trefoil Factor 1 (TFF1) . No significant cross-reactivity or interference between Trefoil Factor 1 (TFF1) and analogues was observed.
Protein Length
81
NCBI Gene Symbol
Cyba
Protein Name
Cytochrome b-245 light chain
Gene Name URL
Cyba
Nucleotide Accession Number
NM_001002992.1

Related Products

CatalogName
OKEH05721-96WTFF1 ELISA Kit (Mouse)
OKCD07094-96WTFF1 ELISA Kit (Mouse)
OKEH02920-96WTFF1 ELISA Kit (Human)
OKCD07093-96WTFF1 ELISA Kit (Human)
OKBB00554TFF1 ELISA Kit (Human) (OKBB00554)
OKCD07094TFF1 ELISA Kit (Mouse) (OKCD07094)
OKBB01134Tff1 ELISA Kit (Mouse) (OKBB01134)
OKEH05721TFF1 ELISA Kit (Mouse) (OKEH05721)
OKAN04999TFF1 ELISA Kit (Human) (OKAN04999)
OKCD07093TFF1 ELISA Kit (Human) (OKCD07093)

Alternative Products

CatalogName
GTR18827273-01Rat TFF1 ELISA Kit
GTR18834254-01Rat TFF1 ELISA Kit (Ready to Use)
GTR18869579-01Rat Trefoil Factor 1 (TFF1) ELISA Kit
ELI-03392rRat Trefoil factor 1,Tff1 ELISA KIT
EK246461Rat TFF1 (Trefoil Factor 1) ELISA Kit
MBS1605447-01Rat Trefoil factor 1, TFF1 ELISA Kit
CRR3094-01TFF1 siRNA (Rat)
46573056TFF1 Adenovirus (Rat)
abx936587-01Rat TFF1 siRNA
abx987441-01Rat TFF1 shRNA Plasmid

Popular Products