RegisterLogin

SNCA ELISA Kit (Mouse)

CAT: 0247-OKCD02898-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD02898-96WSize:96 Well
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Synuclein Alpha (SNCa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Synuclein Alpha (SNCa) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Synuclein, alpha
Gene Aliases
Al; alp; alpha-Syn; alphaSYN; alpha-synuclein; NACP; non-A beta component of AD amyloid; non-A4 component of amyloid; Non-A4 component of amyloid precursor.
Gene ID
20617
Accession Number
NP_001035916.1
Reactivity
Mouse|Mus musculus
Target
May be involved in the regulation of dopamine release and transport.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synuclein Alpha (SNCa) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synuclein Alpha (SNCa) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synuclein Alpha (SNCa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Synuclein Alpha (SNCa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatant and other biological fluids
Detection Range
78-5,000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 95-104 101 EDTA Plasma (n=5) 87-96 90 Heparin Plasma (n=5) 86-105 98 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 95-104 101 Serum (n=5) 95-104 101 EDTA Plasma (n=5) 87-96 90 EDTA Plasma (n=5) 87-96 90 Heparin Plasma (n=5) 86-105 98 Heparin Plasma (n=5) 86-105 98
Sensitivity
28 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-SNCA Microplate 96 Wells (12 x 8 Well strips) SNCA Lyophilized Standard 2 100X Biotinylated SNCA Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-SNCA Microplate 96 Wells (12 x 8 Well strips) Anti-SNCA Microplate 96 Wells (12 x 8 Well strips) SNCA Lyophilized Standard 2 SNCA Lyophilized Standard 2 100X Biotinylated SNCA Detector Antibody 1 x 120 uL 100X Biotinylated SNCA Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Synuclein Alpha (SNCa) . No significant cross-reactivity or interference between Synuclein Alpha (SNCa) and analogues was observed.
Protein Length
140
NCBI Gene Symbol
Snca
Protein Name
Alpha-synuclein
Gene Name URL
Snca
Nucleotide Accession Number
NM_001042451.2

Related Products

CatalogName
OKCD07223-96WSNCA ELISA Kit (Human)
OKEH03912-96WSNCA ELISA Kit (Pig)
OKCD02874-96WSNCA ELISA Kit (Rat)
OKEH00882-96WSNCA ELISA Kit (Human)
OKEH07165-96WSNCA ELISA Kit (Rat)
OKCD07223SNCA ELISA Kit (Human) (OKCD07223)
OKEH07165SNCA ELISA Kit (Rat) (OKEH07165)
OKAN04624SNCA ELISA Kit (Human) (OKAN04624)
OKAN05903SNCA ELISA Kit (Rat) (OKAN05903)
OKCD02874SNCA ELISA Kit (Rat) (OKCD02874)

Alternative Products

CatalogName
EF013899Mouse SNCa ELISA Kit
H1356SNCa (Synuclein (α) Mouse ELISA Kit
B4266Α-synuclein (SNCA) Mouse ELISA Kit
H4201Synuclein α (SNCa) Mouse ELISA Kit
E1401Mo-01Mouse Alpha-Synuclein, SNCA ELISA KIT
E1385MoMouse Snca/Alpha-synuclein ELISA Kit
DLR-SNCa-Mu-96TMouse Synuclein Alpha (SNCa) ELISA Kit
EKU12868Mouse SNCa Mini-sample ELISA Kit
EM23022Mouse SNCa (Synuclein Alpha) ELISA Kit
MBS2508667-01Mouse SNCa (Synuclein, Alpha) ELISA Kit

Popular Products