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SHBG ELISA Kit (Mouse)

CAT: 0247-OKCD02890-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02890-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Sex Hormone Binding Globulin (SHBG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Sex Hormone Binding Globulin (SHBG) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Sex hormone binding globulin
Gene Aliases
ABP; SBP; sex hormone-binding globulin; sex steroid-binding protein; testis-specific androgen-binding protein.
Gene ID
20415
Accession Number
NP_035497.1
Reactivity
Mouse|Mus musculus
Target
Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sex Hormone Binding Globulin (SHBG) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sex Hormone Binding Globulin (SHBG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sex Hormone Binding Globulin (SHBG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sex Hormone Binding Globulin (SHBG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.2-2,000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 86-104 93 EDTA Plasma (n=5) 90-99 95 Heparin Plasma (n=5) 89-98 94 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 86-104 93 Serum (n=5) 86-104 93 EDTA Plasma (n=5) 90-99 95 EDTA Plasma (n=5) 90-99 95 Heparin Plasma (n=5) 89-98 94 Heparin Plasma (n=5) 89-98 94
Sensitivity
13.2 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-SHBG Microplate 96 Wells (12 x 8 Well strips) SHBG Lyophilized Standard 2 100X Biotinylated SHBG Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-SHBG Microplate 96 Wells (12 x 8 Well strips) Anti-SHBG Microplate 96 Wells (12 x 8 Well strips) SHBG Lyophilized Standard 2 SHBG Lyophilized Standard 2 100X Biotinylated SHBG Detector Antibody 1 x 120 uL 100X Biotinylated SHBG Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Sex Hormone Binding Globulin (SHBG) . No significant cross-reactivity or interference between Sex Hormone Binding Globulin (SHBG) and analogues was observed.
Protein Length
403
NCBI Gene Symbol
Shbg
Protein Name
Sex hormone-binding globulin
Gene Name URL
Shbg
Nucleotide Accession Number
NM_011367.2

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