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RIOK1 ELISA Kit (Human)

CAT: 0247-OKCD02849-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02849-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level RIO Kinase 1 (RIOK1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level RIO Kinase 1 (RIOK1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
RIO kinase 1
Gene Aliases
AD034; bA288G3.1; RIO kinase 1; RIO1; RRP10; serine/threonine-protein kinase RIO1.
Gene ID
83732
Accession Number
NP_113668.2
Reactivity
Homo sapiens|Human
Target
Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor (PubMed:22072790) . The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit (By similarity) . Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity) . The catalytic activity regulates its dynamic association with the 40S subunit (By similarity) . In addition to its role in ribosomal biogenesis acts as an adapter protein by recruiting NCL/nucleolin the to PRMT5 complex for its symmetrical methylation (PubMed:21081503) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RIO Kinase 1 (RIOK1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RIO Kinase 1 (RIOK1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RIO Kinase 1 (RIOK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RIO Kinase 1 (RIOK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 82-98 89 EDTA Plasma (n=5) 80-94 84 Heparin Plasma (n=5) 80-103 83 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 82-98 89 Serum (n=5) 82-98 89 EDTA Plasma (n=5) 80-94 84 EDTA Plasma (n=5) 80-94 84 Heparin Plasma (n=5) 80-103 83 Heparin Plasma (n=5) 80-103 83
Sensitivity
0.133 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-RIOK1 Microplate 96 Wells (12 x 8 Well strips) RIOK1 Lyophilized Standard 2 100X Biotinylated RIOK1 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-RIOK1 Microplate 96 Wells (12 x 8 Well strips) Anti-RIOK1 Microplate 96 Wells (12 x 8 Well strips) RIOK1 Lyophilized Standard 2 RIOK1 Lyophilized Standard 2 100X Biotinylated RIOK1 Detector Antibody 1 x 120 uL 100X Biotinylated RIOK1 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of RIO Kinase 1 (RIOK1) . No significant cross-reactivity or interference between RIO Kinase 1 (RIOK1) and analogues was observed.
Protein Length
568
NCBI Gene Symbol
RIOK1
Protein Name
Serine/threonine-protein kinase RIO1
Gene Name URL
RIOK1
Nucleotide Accession Number
NM_031480.2

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