PYGL ELISA Kit (Mouse)

CAT: 0247-OKCD02833-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD02833-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Glycogen Phosphorylase, Liver (PYGL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Glycogen Phosphorylase, Liver (PYGL) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Liver glycogen phosphorylase
Gene Aliases
Glycogen phosphorylase, liver form.
Gene ID
110095
Accession Number
NP_573461.2
Reactivity
Mouse|Mus musculus
Target
Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycogen Phosphorylase, Liver (PYGL) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycogen Phosphorylase, Liver (PYGL) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycogen Phosphorylase, Liver (PYGL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glycogen Phosphorylase, Liver (PYGL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 85-104 88 EDTA Plasma (n=5) 80-101 93 Heparin Plasma (n=5) 99-105 102 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 85-104 88 Serum (n=5) 85-104 88 EDTA Plasma (n=5) 80-101 93 EDTA Plasma (n=5) 80-101 93 Heparin Plasma (n=5) 99-105 102 Heparin Plasma (n=5) 99-105 102
Sensitivity
0.065 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-PYGL Microplate 96 Wells (12 x 8 Well strips) PYGL Lyophilized Standard 2 100X Biotinylated PYGL Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-PYGL Microplate 96 Wells (12 x 8 Well strips) Anti-PYGL Microplate 96 Wells (12 x 8 Well strips) PYGL Lyophilized Standard 2 PYGL Lyophilized Standard 2 100X Biotinylated PYGL Detector Antibody 1 x 120 uL 100X Biotinylated PYGL Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Glycogen Phosphorylase, Liver (PYGL) . No significant cross-reactivity or interference between Glycogen Phosphorylase, Liver (PYGL) and analogues was observed.
Protein Length
850
NCBI Gene Symbol
Pygl
Protein Name
Glycogen phosphorylase, liver form
Gene Name URL
Pygl
Nucleotide Accession Number
NM_133198.2

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