PROZ ELISA Kit (Mouse)

CAT: 0247-OKCD02825-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD02825-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Protein Z (PROZ) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Protein Z (PROZ) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Protein Z, vitamin K-dependent plasma glycoprotein
Gene Aliases
1300015B06Rik; vitamin K-dependent protein Z.
Gene ID
66901
Accession Number
NP_080110.1
Reactivity
Mouse|Mus musculus
Target
Appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. Inhibits activity of the coagulation protease factor Xa in the presence of SERPINA10, calcium and phospholipids (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Z (PROZ) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Z (PROZ) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Z (PROZ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Z (PROZ) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Plasma
Detection Range
0.156-10ng/mL
Recovery
Matrix Recovery range (%) Average (%) _x005F sodium citrate plasma (n=5) 88-105 96 _x005F Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) sodium citrate plasma (n=5) 88-105 96 sodium citrate plasma (n=5) 88-105 96
Sensitivity
0.067 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount _x005F Anti-PROZ Microplate 96 Wells (12 x 8 Well strips) _x005F PROZ Lyophilized Standard 2 _x005F 100X Biotinylated PROZ Detector Antibody 1 x 120 uL _x005F 100X Avidin-HRP Conjugate 1 x 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F Stop Solution 1 x 6 mL _x005F TMB Substrate 1 x 9 mL _x005F Component Amount Component Amount Anti-PROZ Microplate 96 Wells (12 x 8 Well strips) Anti-PROZ Microplate 96 Wells (12 x 8 Well strips) PROZ Lyophilized Standard 2 PROZ Lyophilized Standard 2 100X Biotinylated PROZ Detector Antibody 1 x 120 uL 100X Biotinylated PROZ Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Protein Z (PROZ) ._x005F No significant cross-reactivity or interference between Protein Z (PROZ) and analogues was observed.
Protein Length
399
NCBI Gene Symbol
Proz
Protein Name
Vitamin K-dependent protein Z
Gene Name URL
Proz
Nucleotide Accession Number
NM_025834.3

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