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NOX4 ELISA Kit (Mouse)

CAT: 0247-OKCD02767-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02767-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level NADPH Oxidase 4 (NOX4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level NADPH Oxidase 4 (NOX4) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
NADPH oxidase 4
Gene Aliases
AI648021; kidney oxidase-1; kidney superoxide-producing NADPH oxidase; KOX-1; NADPH oxidase 4; renal NAD (P) H-oxidase; superoxide-generating NADPH oxidase 4.
Gene ID
50490
Accession Number
NP_056575.1
Reactivity
Mouse|Mus musculus
Target
Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NADPH Oxidase 4 (NOX4) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NADPH Oxidase 4 (NOX4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NADPH Oxidase 4 (NOX4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NADPH Oxidase 4 (NOX4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.058 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-NOX4 Microplate 96 Wells (12 x 8 Well strips) NOX4 Lyophilized Standard 2 100X Biotinylated NOX4 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-NOX4 Microplate 96 Wells (12 x 8 Well strips) Anti-NOX4 Microplate 96 Wells (12 x 8 Well strips) NOX4 Lyophilized Standard 2 NOX4 Lyophilized Standard 2 100X Biotinylated NOX4 Detector Antibody 1 x 120 uL 100X Biotinylated NOX4 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4) . No significant cross-reactivity or interference between Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4) and analogues was observed.
Protein Length
578
NCBI Gene Symbol
Nox4
Protein Name
NADPH oxidase 4
Gene Name URL
Nox4
Nucleotide Accession Number
NM_015760.5

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