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MRC1 ELISA Kit (Mouse)

CAT: 0247-OKCD02731-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02731-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Mannose Receptor C Type 1 (MRC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Mannose Receptor C Type 1 (MRC1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Mannose receptor, C type 1
Gene Aliases
AW259686; CD206; M; macrophage mannose receptor 1; MMR; MR.
Gene ID
17533
Accession Number
NP_032651.2
Reactivity
Mouse|Mus musculus
Target
Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Receptor C Type 1 (MRC1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Receptor C Type 1 (MRC1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Receptor C Type 1 (MRC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mannose Receptor C Type 1 (MRC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Detection Range
15.6-1,000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 93-101 97 EDTA Plasma (n=5) 79-95 87 Heparin Plasma (n=5) 96-104 99 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 93-101 97 Serum (n=5) 93-101 97 EDTA Plasma (n=5) 79-95 87 EDTA Plasma (n=5) 79-95 87 Heparin Plasma (n=5) 96-104 99 Heparin Plasma (n=5) 96-104 99
Sensitivity
6.6 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-MRC1 Microplate 96 Wells (12 x 8 Well strips) MRC1 Lyophilized Standard 2 100X Biotinylated MRC1 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-MRC1 Microplate 96 Wells (12 x 8 Well strips) Anti-MRC1 Microplate 96 Wells (12 x 8 Well strips) MRC1 Lyophilized Standard 2 MRC1 Lyophilized Standard 2 100X Biotinylated MRC1 Detector Antibody 1 x 120 uL 100X Biotinylated MRC1 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Mannose Receptor C Type 1 (MRC1) . No significant cross-reactivity or interference between Mannose Receptor C Type 1 (MRC1) and analogues was observed.
Protein Length
1456
NCBI Gene Symbol
Mrc1
Protein Name
Macrophage mannose receptor 1
Gene Name URL
Mrc1
Nucleotide Accession Number
NM_008625.2

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