Products for Research Use Only
RegisterLogin

PSMB8 ELISA Kit (Human)

CAT: 0247-OKCD02695-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKCD02695-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Large Multifunctional Peptidase 7 (LMP7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Large Multifunctional Peptidase 7 (LMP7) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Proteasome 20S subunit beta 8
Gene Aliases
ALDD; D6S216; D6S216E; JMP; LMP7; low molecular mass protein 7; low molecular weight protein 7; macropain subunit C13; multicatalytic endopeptidase complex subunit C13; NKJO; PRAAS1; protease component C13; proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7) ; proteasome catalytic subunit 3i; proteasome component C13; proteasome subunit beta 5i; proteasome subunit beta 8; proteasome subunit beta type-8; Proteasome subunit beta-5i; proteasome subunit Y2; proteasome-related gene 7; PSMB5i; really interesting new gene 10 protein; RING10.
Gene ID
5696
Accession Number
NP_004150.1
Reactivity
Homo sapiens|Human
Target
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Large Multifunctional Peptidase 7 (LMP7) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Large Multifunctional Peptidase 7 (LMP7) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Large Multifunctional Peptidase 7 (LMP7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Large Multifunctional Peptidase 7 (LMP7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.117 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-PSMB8 Microplate 96 Wells (12 x 8 Well strips) PSMB8 Lyophilized Standard 2 100X Biotinylated PSMB8 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-PSMB8 Microplate 96 Wells (12 x 8 Well strips) Anti-PSMB8 Microplate 96 Wells (12 x 8 Well strips) PSMB8 Lyophilized Standard 2 PSMB8 Lyophilized Standard 2 100X Biotinylated PSMB8 Detector Antibody 1 x 120 uL 100X Biotinylated PSMB8 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Large Multifunctional Peptidase 7 (LMP7) . No significant cross-reactivity or interference between Large Multifunctional Peptidase 7 (LMP7) and analogues was observed.
Protein Length
276
NCBI Gene Symbol
PSMB8
Protein Name
Proteasome subunit beta type-8
Gene Name URL
PSMB8
Nucleotide Accession Number
NM_004159.4

Related Products

CatalogName
OKEH03975-96WPSMB8 ELISA Kit (Dog)
OKEH03975PSMB8 ELISA Kit (Dog) (OKEH03975)
OAGA01026-100ULPSMB8 antibody
OACD05288-100ULPSMB8 Antibody
OAAN02231-50ULPSMB8 Antibody
OACD05289-100ULPSMB8 Antibody
OACD05289-1MLPSMB8 Antibody
OAAN02231-200ULPSMB8 Antibody
ARP88638_P050Anti-PSMB8
OACD05288-1MLPSMB8 Antibody

Alternative Products

CatalogName
GTR10444902Human Proteasome subunit β type 8 (PSMB8) ELISA kit
G2308Proteasome subunit β type-8 (PSMB8) Human ELISA Kit
MBS2805674-01Human Proteasome subunit beta type-8 (PSMB8) ELISA Kit
E2074HuHuman PSMB8/Proteasome subunit beta type-8 ELISA Kit
abx515623Human Low Molecular Mass Protein 7 (PSMB8) ELISA Kit
MBS7214214-01Human Proteasome subunit beta type 8 (PSMB8) ELISA Kit
ELI-15688hHuman Proteasome subunit beta type-8, PSMB8 ELISA KIT
MBS2509694-01Human LMP7/PSMB8 (Low molecular mass protein 7/Proteasome subunit beta type-8) ELISA Kit
GTR18856651-01Human Low molecular mass protein 7/Proteasome subunit beta type-8 (LMP7/PSMB8) ELISA Kit
E-EL-H1209ELISA kit for Human LMP7/PSMB8 (Low molecular mass protein 7/Proteasome subunit beta type-8)

Popular Products