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LIPC ELISA Kit (Mouse)

CAT: 0247-OKCD02691-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02691-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Lipase, Hepatic (LIPC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Lipase, Hepatic (LIPC) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Lipase, hepatic
Gene Aliases
AI256194; H; hepatic lipase; hepatic triacylglycerol lipase; HL; Hpl; lipase member C; lysophospholipase; phospholipase A1.
Gene ID
15450
Accession Number
NP_001311401.1
Reactivity
Mouse|Mus musculus
Target
Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Hepatic (LIPC) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase, Hepatic (LIPC) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Hepatic (LIPC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lipase, Hepatic (LIPC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, other biological fluids
Detection Range
3.12-200ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 86-96 90 EDTA Plasma (n=5) 95-104 101 Heparin Plasma (n=5) 93-105 99 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 86-96 90 Serum (n=5) 86-96 90 EDTA Plasma (n=5) 95-104 101 EDTA Plasma (n=5) 95-104 101 Heparin Plasma (n=5) 93-105 99 Heparin Plasma (n=5) 93-105 99
Sensitivity
0.126 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-LIPC Microplate 96 Wells (12 x 8 Well strips) LIPC Lyophilized Standard 2 100X Biotinylated LIPC Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-LIPC Microplate 96 Wells (12 x 8 Well strips) Anti-LIPC Microplate 96 Wells (12 x 8 Well strips) LIPC Lyophilized Standard 2 LIPC Lyophilized Standard 2 100X Biotinylated LIPC Detector Antibody 1 x 120 uL 100X Biotinylated LIPC Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Lipase, Hepatic (LIPC) . No significant cross-reactivity or interference between Lipase, Hepatic (LIPC) and analogues was observed. Recovery
Protein Length
510
NCBI Gene Symbol
Lipc
Protein Name
Hepatic triacylglycerol lipase
Gene Name URL
Lipc
Nucleotide Accession Number
NM_001324472.1

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