LECT2 ELISA Kit (Mouse)

CAT: 0247-OKCD02685-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD02685-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Leukocyte Cell Derived Chemotaxin 2 (LECT2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Leukocyte Cell Derived Chemotaxin 2 (LECT2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Leukocyte cell-derived chemotaxin 2
Gene Aliases
ChM-II; chondromodulin II; LECT-2; leukocyte cell-derived chemotaxin-2.
Gene ID
16841
Accession Number
NP_034832.2
Reactivity
Mouse|Mus musculus
Target
Has a neutrophil chemotactic activity. Also a positive regulator of chondrocyte proliferation.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukocyte Cell Derived Chemotaxin 2 (LECT2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leukocyte Cell Derived Chemotaxin 2 (LECT2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukocyte Cell Derived Chemotaxin 2 (LECT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Leukocyte Cell Derived Chemotaxin 2 (LECT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
78-5,000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 90-103 97 EDTA Plasma (n=5) 97-105 102 Heparin Plasma (n=5) 93-105 96 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 90-103 97 Serum (n=5) 90-103 97 EDTA Plasma (n=5) 97-105 102 EDTA Plasma (n=5) 97-105 102 Heparin Plasma (n=5) 93-105 96 Heparin Plasma (n=5) 93-105 96
Sensitivity
32 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-LECT2 Microplate 96 Wells (12 x 8 Well strips) LECT2 Lyophilized Standard 2 100X Biotinylated LECT2 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-LECT2 Microplate 96 Wells (12 x 8 Well strips) Anti-LECT2 Microplate 96 Wells (12 x 8 Well strips) LECT2 Lyophilized Standard 2 LECT2 Lyophilized Standard 2 100X Biotinylated LECT2 Detector Antibody 1 x 120 uL 100X Biotinylated LECT2 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Leukocyte Cell Derived Chemotaxin 2 (LECT2) . No significant cross-reactivity or interference between Leukocyte Cell Derived Chemotaxin 2 (LECT2) and analogues was observed.
Protein Length
151
NCBI Gene Symbol
Lect2
Protein Name
Leukocyte cell-derived chemotaxin-2
Gene Name URL
Lect2
Nucleotide Accession Number
NM_010702.2

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