KRT19 ELISA Kit (Mouse)

CAT: 0247-OKCD02676-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD02676-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Cytokeratin 19 (CK19) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Cytokeratin 19 (CK19) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Keratin 19
Gene Aliases
AI663979; CK-19; cytokeratin 19; Cytokeratin-19; End; EndoC; K19; keratin complex 1, acidic, gene 19; keratin, type I cytoskeletal 19; Keratin-19; Krt-1.1; Krt-1.19; Krt1-1; Krt1-19.
Gene ID
16669
Accession Number
NP_032497.1
Reactivity
Mouse|Mus musculus
Target
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytokeratin 19 (CK19) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytokeratin 19 (CK19) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytokeratin 19 (CK19), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytokeratin 19 (CK19) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
7.8-500pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 91-98 94 EDTA Plasma (n=5) 78-102 81 Heparin Plasma (n=5) 98-105 102 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 91-98 94 Serum (n=5) 91-98 94 EDTA Plasma (n=5) 78-102 81 EDTA Plasma (n=5) 78-102 81 Heparin Plasma (n=5) 98-105 102 Heparin Plasma (n=5) 98-105 102
Sensitivity
13.6 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-KRT19 Microplate 96 Wells (12 x 8 Well strips) KRT19 Lyophilized Standard 2 100X Biotinylated KRT19 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-KRT19 Microplate 96 Wells (12 x 8 Well strips) Anti-KRT19 Microplate 96 Wells (12 x 8 Well strips) KRT19 Lyophilized Standard 2 KRT19 Lyophilized Standard 2 100X Biotinylated KRT19 Detector Antibody 1 x 120 uL 100X Biotinylated KRT19 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Keratin 19 (KRT19) . No significant cross-reactivity or interference between Keratin 19 (KRT19) and analogues was observed.
Protein Length
403
NCBI Gene Symbol
Krt19
Protein Name
Keratin, type I cytoskeletal 19
Gene Name URL
Krt19
Nucleotide Accession Number
NM_008471.3

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