ITIH4 ELISA Kit (Mouse)

• Reproducibility :

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Inter Alpha-Globulin Inhibitor H4 (ITIH4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Inter Alpha-Globulin Inhibitor H4 (ITIH4) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name :

  Inter alpha-trypsin inhibitor, heavy chain 4

• Gene Aliases :

  Inter alpha-trypsin inhibitor, heavy chain 4; inter-alpha-inhibitor heavy chain 4; ITI heavy chain H4; Itih; Itih-4; ITI-HC4; PK-120.

• Gene ID :

  16427

• Accession Number :

  NP_001152771.1

• Reactivity :

  Mouse|Mus musculus

• Target :

  Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.

• Type :

  ELISA Kit

• Applications :

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method :

  Colorimetric

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inter Alpha-Globulin Inhibitor H4 (ITIH4) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inter Alpha-Globulin Inhibitor H4 (ITIH4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inter Alpha-Globulin Inhibitor H4 (ITIH4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Inter Alpha-Globulin Inhibitor H4 (ITIH4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Serum, plasma and other biological fluids

• Detection Range :

  7.8-500ng/mL

• Recovery :

  Matrix Recovery range (%) Average (%) Serum (n=5) 80-103 95 EDTA Plasma (n=5) 89-102 95 Heparin Plasma (n=5) 82-97 88 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 80-103 95 Serum (n=5) 80-103 95 EDTA Plasma (n=5) 89-102 95 EDTA Plasma (n=5) 89-102 95 Heparin Plasma (n=5) 82-97 88 Heparin Plasma (n=5) 82-97 88

• Sensitivity :

  3.7 ng/mL

• Reconstitution :

  2°C to 8°C|-20°C

• Components :

  Component Amount Anti-ITIH4 Microplate 96 Wells (12 x 8 Well strips) ITIH4 Lyophilized Standard 2 100X Biotinylated ITIH4 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-ITIH4 Microplate 96 Wells (12 x 8 Well strips) Anti-ITIH4 Microplate 96 Wells (12 x 8 Well strips) ITIH4 Lyophilized Standard 2 ITIH4 Lyophilized Standard 2 100X Biotinylated ITIH4 Detector Antibody 1 x 120 uL 100X Biotinylated ITIH4 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

• Specificity :

  This assay has high sensitivity and excellent specificity for detection of Inter Alpha-Globulin Inhibitor H4 (ITIH4) . No significant cross-reactivity or interference between Inter Alpha-Globulin Inhibitor H4 (ITIH4) and analogues was observed.

• Protein Length :

  942

• NCBI Gene Symbol :

  Itih4

• Protein Name :

  Inter alpha-trypsin inhibitor, heavy chain 4

• Gene Name URL :

  Itih4

• CAS Number :

  7732-18-5

• Nucleotide Accession Number :

  NM_001159299.2