IGFBP4 ELISA Kit (Cattle)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 4 (IGFBP4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 4 (IGFBP4) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

7732-18-5

Insulin like growth factor binding protein 4

IBP-4; IGF-binding protein 4; IGFBP-4; insulin-like growth factor-binding protein 4.

282262

NP_776982.1

Bos taurus|Bovine

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

ELISA Kit

Enzyme-linked Immunosorbent assay-Competitive

Colorimetric

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Insulin Like Growth Factor Binding Protein 4 (IGFBP4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Insulin Like Growth Factor Binding Protein 4 (IGFBP4) and unlabeled Insulin Like Growth Factor Binding Protein 4 (IGFBP4) (Standards or samples) with the pre-coated antibody specific to Insulin Like Growth Factor Binding Protein 4 (IGFBP4) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Insulin Like Growth Factor Binding Protein 4 (IGFBP4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Insulin Like Growth Factor Binding Protein 4 (IGFBP4) in the sample.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, plasma and other biological fluids

74.07-6000pg/mL

Matrix Recovery range (%) Average (%) Serum (n=5) 81-94 87 EDTA Plasma (n=5) 78-93 89 Heparin Plasma (n=5) 86-99 93 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 81-94 87 Serum (n=5) 81-94 87 EDTA Plasma (n=5) 78-93 89 EDTA Plasma (n=5) 78-93 89 Heparin Plasma (n=5) 86-99 93 Heparin Plasma (n=5) 86-99 93

< 29.62 pg/mL

2°C to 8°C|-20°C

258 kDa

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This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 4 (IGFBP4) . No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 4 (IGFBP4) and analogues was observed.

IGFBP4

Insulin-like growth factor-binding protein 4

IGFBP4