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ICAM4 ELISA Kit (Mouse)

CAT: 0247-OKCD02608-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02608-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Intercellular Adhesion Molecule 4 (ICAM4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Intercellular Adhesion Molecule 4 (ICAM4) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Intercellular adhesion molecule 4, Landsteiner-Wiener blood group
Gene Aliases
1810015M19Rik; Cd242; ICAM-4; intercellular adhesion molecule 4; LW glycoprotein.
Gene ID
78369
Accession Number
NP_076381.1
Reactivity
Mouse|Mus musculus
Target
Adhesion molecule that binds to leukocyte adhesion LFA-1 protein LFA-1 (integrin alpha-L/beta-2) . ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins (By similarity) . Isoform 2 may modulate binding of membrane-associated ICAM4.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Intercellular Adhesion Molecule 4 (ICAM4) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Intercellular Adhesion Molecule 4 (ICAM4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Intercellular Adhesion Molecule 4 (ICAM4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Intercellular Adhesion Molecule 4 (ICAM4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Matrix Recovery range (%) Average (%) 90-104 97 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) 90-104 97 90-104 97
Sensitivity
0.059 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-ICAM4 Microplate 96 Wells (12 x 8 Well strips) ICAM4 Lyophilized Standard 2 100X Biotinylated ICAM4 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-ICAM4 Microplate 96 Wells (12 x 8 Well strips) Anti-ICAM4 Microplate 96 Wells (12 x 8 Well strips) ICAM4 Lyophilized Standard 2 ICAM4 Lyophilized Standard 2 100X Biotinylated ICAM4 Detector Antibody 1 x 120 uL 100X Biotinylated ICAM4 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Intercellular Adhesion Molecule 4 (ICAM4) . No significant cross-reactivity or interference between Intercellular Adhesion Molecule 4 (ICAM4) and analogues was observed.
Protein Length
262
NCBI Gene Symbol
Icam4
Protein Name
Intercellular adhesion molecule 4
Gene Name URL
Icam4
Nucleotide Accession Number
NM_023892.2

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