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HSPB1 ELISA Kit (Mouse)

CAT: 0247-OKCD02600-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02600-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Heat Shock Protein 27 (Hsp27) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Heat Shock Protein 27 (Hsp27) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Heat shock protein 1
Gene Aliases
27kDa; growth-related 25 kDa protein; heat shock 25 kDa protein; heat shock 27 kDa protein; heat shock 27kDa protein 1; heat shock protein beta-1; heat shock protein, 25 kDa; Hsp; HSP 25; HSP 27; Hsp25; p25; truncated hsp25.
Gene ID
15507
Accession Number
NP_038588.2
Reactivity
Mouse|Mus musculus
Target
Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization (PubMed:17661394) . Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 27 (Hsp27) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 27 (Hsp27) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 27 (Hsp27), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heat Shock Protein 27 (Hsp27) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.78-50ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 95-102 98 EDTA Plasma (n=5) 88-97 93 Heparin Plasma (n=5) 96-103 99 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 95-102 98 Serum (n=5) 95-102 98 EDTA Plasma (n=5) 88-97 93 EDTA Plasma (n=5) 88-97 93 Heparin Plasma (n=5) 96-103 99 Heparin Plasma (n=5) 96-103 99
Sensitivity
0.32 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-HSPB1 Microplate 96 Wells (12 x 8 Well strips) HSPB1 Lyophilized Standard 2 100X Biotinylated HSPB1 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-HSPB1 Microplate 96 Wells (12 x 8 Well strips) Anti-HSPB1 Microplate 96 Wells (12 x 8 Well strips) HSPB1 Lyophilized Standard 2 HSPB1 Lyophilized Standard 2 100X Biotinylated HSPB1 Detector Antibody 1 x 120 uL 100X Biotinylated HSPB1 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Heat Shock 27kDa Protein 1 (HSPB1) . No significant cross-reactivity or interference between Heat Shock 27kDa Protein 1 (HSPB1) and analogues was observed
Protein Length
209
NCBI Gene Symbol
Hspb1
Protein Name
Heat shock protein beta-1
Gene Name URL
Hspb1
Nucleotide Accession Number
NM_013560.2

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