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HSD17B10 ELISA Kit (Mouse)

CAT: 0247-OKCD02595-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02595-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Aliases
17-beta-hydroxysteroid dehydrogenase 10;3-hydroxy-2-methylbutyryl-CoA dehydrogenase;3-hydroxyacyl-CoA dehydrogenase type II; Endoplasmic reticulum-associated amyloid beta-peptide-binding protein; Mitochondrial ribonuclease P protein 2; Type II HADH.
Accession Number
NP_058043.3
Reactivity
Mouse|Mus musculus
Target
Mitochondrial dehydrogenase that catalyzes the beta-oxidation at position 17 of androgens and estrogens and has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone (By similarity) . Catalyzes the third step in the beta-oxidation of fatty acids (By similarity) . Carries out oxidative conversions of 7-alpha-OH and 7-beta-OH bile acids (By similarity) . Also exhibits 20-beta-OH and 21-OH dehydrogenase activities with C21 steroids (By similarity) . By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD) (By similarity) . Essential for structural and functional integrity of mitochondria (PubMed:20077426) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.063 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-HSD17B10 Microplate 96 Wells (12 x 8 Well strips) HSD17B10 Lyophilized Standard 2 100X Biotinylated HSD17B10 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-HSD17B10 Microplate 96 Wells (12 x 8 Well strips) Anti-HSD17B10 Microplate 96 Wells (12 x 8 Well strips) HSD17B10 Lyophilized Standard 2 HSD17B10 Lyophilized Standard 2 100X Biotinylated HSD17B10 Detector Antibody 1 x 120 uL 100X Biotinylated HSD17B10 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) . No significant cross-reactivity or interference between 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) and analogues was observed.
Protein Length
261
NCBI Gene Symbol
HSD17B10
Protein Name
3-hydroxyacyl-CoA dehydrogenase type-2
Gene Name URL
HSD17B10
Nucleotide Accession Number
NM_016763.2

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