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ENPEP ELISA Kit (Mouse)

CAT: 0247-OKCD02574-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02574-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Aminopeptidase A (ENPEP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Aminopeptidase A (ENPEP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Glutamyl aminopeptidase
Gene Aliases
6030431M22Rik; aminopeptidase A; APA; AP-A; Bp-1/6C; Bp-1/6C3; BP-1/6C3 antigen; EAP; glutamyl aminopeptidase; Ly5; Ly-5; Ly51; Ly-51.
Gene ID
13809
Accession Number
NP_031960.1
Reactivity
Mouse|Mus musculus
Target
Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aminopeptidase A (ENPEP) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aminopeptidase A (ENPEP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aminopeptidase A (ENPEP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aminopeptidase A (ENPEP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 97-104 101 EDTA Plasma (n=5) 91-98 94 Heparin Plasma (n=5) 86-98 93 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 97-104 101 Serum (n=5) 97-104 101 EDTA Plasma (n=5) 91-98 94 EDTA Plasma (n=5) 91-98 94 Heparin Plasma (n=5) 86-98 93 Heparin Plasma (n=5) 86-98 93
Sensitivity
0.062 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-ENPEP Microplate 96 Wells (12 x 8 Well strips) ENPEP Lyophilized Standard 2 100X Biotinylated ENPEP Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-ENPEP Microplate 96 Wells (12 x 8 Well strips) Anti-ENPEP Microplate 96 Wells (12 x 8 Well strips) ENPEP Lyophilized Standard 2 ENPEP Lyophilized Standard 2 100X Biotinylated ENPEP Detector Antibody 1 x 120 uL 100X Biotinylated ENPEP Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Glutamyl Aminopeptidase (GluAP) . No significant cross-reactivity or interference between Glutamyl Aminopeptidase (GluAP) and analogues was observed.
Protein Length
945
NCBI Gene Symbol
Enpep
Protein Name
Glutamyl aminopeptidase
Gene Name URL
Enpep
Nucleotide Accession Number
NM_007934.3

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