ERBB3 ELISA Kit (Mouse)

CAT: 0247-OKCD02526-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD02526-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Erb-b2 receptor tyrosine kinase 3
Gene Aliases
Avian erythroblastosis oncogene B 3 receptor; C76256; c-erbB-3; Erbb; Erbb-; Erbb-3; Erbb3r; glial growth factor receptor; HER; Her3; proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3.
Gene ID
13867
Accession Number
NP_034283.1
Reactivity
Mouse|Mus musculus
Target
Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. May also be activated by CSPG5.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
7.8-500pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 79-96 87 EDTA Plasma (n=5) 82-102 92 Heparin Plasma (n=5) 91-101 98 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 79-96 87 Serum (n=5) 79-96 87 EDTA Plasma (n=5) 82-102 92 EDTA Plasma (n=5) 82-102 92 Heparin Plasma (n=5) 91-101 98 Heparin Plasma (n=5) 91-101 98
Sensitivity
3.4 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-ERBB3 Microplate 96 Wells (12 x 8 Well strips) ERBB3 Lyophilized Standard 2 100X Biotinylated ERBB3 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-ERBB3 Microplate 96 Wells (12 x 8 Well strips) Anti-ERBB3 Microplate 96 Wells (12 x 8 Well strips) ERBB3 Lyophilized Standard 2 ERBB3 Lyophilized Standard 2 100X Biotinylated ERBB3 Detector Antibody 1 x 120 uL 100X Biotinylated ERBB3 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) . No significant cross-reactivity or interference between V-Erb B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (ErbB3) and analogues was observed.
Protein Length
1339
NCBI Gene Symbol
Erbb3
Protein Name
Receptor tyrosine-protein kinase erbB-3
Gene Name URL
Erbb3
Nucleotide Accession Number
NM_010153.1

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