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ECM1 ELISA Kit (Mouse)

CAT: 0247-OKCD02510-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02510-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Extracellular Matrix Protein 1 (ECM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Extracellular Matrix Protein 1 (ECM1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Extracellular matrix protein 1
Gene Aliases
AI663821; extracellular matrix protein 1; p8; p85; secretory component p85.
Gene ID
13601
Accession Number
NP_031925.2
Reactivity
Mouse|Mus musculus
Target
Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Extracellular Matrix Protein 1 (ECM1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Extracellular Matrix Protein 1 (ECM1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Extracellular Matrix Protein 1 (ECM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Extracellular Matrix Protein 1 (ECM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
46.88-3000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 81-102 90 EDTA Plasma (n=5) 80-91 86 Heparin Plasma (n=5) 88-96 92 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 81-102 90 Serum (n=5) 81-102 90 EDTA Plasma (n=5) 80-91 86 EDTA Plasma (n=5) 80-91 86 Heparin Plasma (n=5) 88-96 92 Heparin Plasma (n=5) 88-96 92
Sensitivity
14.45 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-ECM1 Microplate 96 Wells (12 x 8 Well strips) ECM1 Lyophilized Standard 2 100X Biotinylated ECM1 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-ECM1 Microplate 96 Wells (12 x 8 Well strips) Anti-ECM1 Microplate 96 Wells (12 x 8 Well strips) ECM1 Lyophilized Standard 2 ECM1 Lyophilized Standard 2 100X Biotinylated ECM1 Detector Antibody 1 x 120 uL 100X Biotinylated ECM1 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Extracellular Matrix Protein 1 (ECM1) . No significant cross-reactivity or interference between Extracellular Matrix Protein 1 (ECM1) and analogues was observed
Protein Length
559
NCBI Gene Symbol
Ecm1
Protein Name
Extracellular matrix protein 1
Gene Name URL
Ecm1
Nucleotide Accession Number
NM_007899.2

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