DDIT3 ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level DNA Damage Inducible Transcript 3 (DDIT3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level DNA Damage Inducible Transcript 3 (DDIT3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  DNA-damage inducible transcript 3

• Gene Aliases:

  AltDDIT3; alternative DDIT3 proteins; C/EBP homoologous protein 10; C/EBP zeta; C/EBP-homologous protein; c/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; ch; chop; CHOP-; CHOP10; CHOP-10; DDIT-3; DNA damage-inducible transcript 3 protein; gadd15; gadd153; growth arrest and DNA-damage-inducible protein GADD153.

• Gene ID:

  13198

• Accession Number:

  NP_001277112.1

• Reactivity:

  Mouse|Mus musculus

• Target:

  Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG) . Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response. Acts as a major regulator of postnatal neovascularization through regulation of endothelial nitric oxide synthase (NOS3) -related signaling.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Damage Inducible Transcript 3 (DDIT3) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DNA Damage Inducible Transcript 3 (DDIT3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA Damage Inducible Transcript 3 (DDIT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DNA Damage Inducible Transcript 3 (DDIT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Tissue homogenates, cell lysates and other biological fluids

• Detection Range:

  0.156-10ng/mL

• Sensitivity:

  < 0.056 ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-DDIT3 Microplate 96 Wells (12 x 8 Well strips) DDIT3 Lyophilized Standard 2 100X Biotinylated DDIT3 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-DDIT3 Microplate 96 Wells (12 x 8 Well strips) Anti-DDIT3 Microplate 96 Wells (12 x 8 Well strips) DDIT3 Lyophilized Standard 2 DDIT3 Lyophilized Standard 2 100X Biotinylated DDIT3 Detector Antibody 1 x 120 uL 100X Biotinylated DDIT3 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of DNA Damage Inducible Transcript 3 (DDIT3) ._x005F No significant cross-reactivity or interference between DNA Damage Inducible Transcript 3 (DDIT3) and analogues was observed.

• Protein Length:

  168

• NCBI Gene Symbol:

  Ddit3

• Protein Name:

  DNA damage-inducible transcript 3 protein

• Gene Name URL:

  Ddit3

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001290183.1