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CXCR4 ELISA Kit (Mouse)

CAT: 0247-OKCD02480-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02480-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Chemokine C-X-C-Motif Receptor 4 (CXCR4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Chemokine C-X-C-Motif Receptor 4 (CXCR4) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Chemokine (C-X-C motif) receptor 4
Gene Aliases
B2b220C; b2b220Clo; CD184; chemokine (C-X-C) receptor 4; chemokine receptor 4; Cmka; Cmkar4; C-X-C chemokine receptor type 4; CXCR-4; CXC-R4; fu; fusin; LESTR; leukocyte-derived seven transmembrane domain receptor; leukocyte-expressed seven-transmembrane-domain; PB-C; PB-CKR; PBSF/SDF-1; pre-B-cell-derived chemokine receptor; Sdf; SDF-1 receptor; Sdf1r; stromal cell-derived factor 1 receptor.
Gene ID
12767
Accession Number
NP_034041.2
Reactivity
Mouse|Mus musculus
Target
Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes (By similarity) . Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X-C-Motif Receptor 4 (CXCR4) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-X-C-Motif Receptor 4 (CXCR4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X-C-Motif Receptor 4 (CXCR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chemokine C-X-C-Motif Receptor 4 (CXCR4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
78-5,000pg/mL
Sensitivity
31 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-CXCR4 Microplate 96 Wells (12 x 8 Well strips) CXCR4 Lyophilized Standard 2 100X Biotinylated CXCR4 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-CXCR4 Microplate 96 Wells (12 x 8 Well strips) Anti-CXCR4 Microplate 96 Wells (12 x 8 Well strips) CXCR4 Lyophilized Standard 2 CXCR4 Lyophilized Standard 2 100X Biotinylated CXCR4 Detector Antibody 1 x 120 uL 100X Biotinylated CXCR4 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Chemokine C-X-C-Motif Receptor 4 (CXCR4) . No significant cross-reactivity or interference between Chemokine C-X-C-Motif Receptor 4 (CXCR4) and analogues was observed.
Protein Length
359
NCBI Gene Symbol
Cxcr4
Protein Name
C-X-C chemokine receptor type 4
Gene Name URL
Cxcr4
Nucleotide Accession Number
NM_009911.3

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