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CTNNB1 ELISA Kit (Mouse)

CAT: 0247-OKCD02471-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02471-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Beta Catenin (Beta-catenin) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Beta Catenin (Beta-catenin) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Catenin (cadherin associated protein), beta 1
Gene Aliases
Beta-catenin; Bfc; Cat; catenin beta-1; Catnb; Mesc.
Gene ID
12387
Accession Number
NP_001159374.1
Reactivity
Mouse|Mus musculus
Target
Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (By similarity) . Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (PubMed:21325504) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta Catenin (Beta-catenin) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta Catenin (Beta-catenin) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta Catenin (Beta-catenin), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Beta Catenin (Beta-catenin) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 88-97 93 EDTA Plasma (n=5) 95-103 99 Heparin Plasma (n=5) 81-97 88 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 88-97 93 Serum (n=5) 88-97 93 EDTA Plasma (n=5) 95-103 99 EDTA Plasma (n=5) 95-103 99 Heparin Plasma (n=5) 81-97 88 Heparin Plasma (n=5) 81-97 88
Sensitivity
5.9 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-CTNNB1 Microplate 96 Wells (12 x 8 Well strips) CTNNB1 Lyophilized Standard 2 100X Biotinylated CTNNB1 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-CTNNB1 Microplate 96 Wells (12 x 8 Well strips) Anti-CTNNB1 Microplate 96 Wells (12 x 8 Well strips) CTNNB1 Lyophilized Standard 2 CTNNB1 Lyophilized Standard 2 100X Biotinylated CTNNB1 Detector Antibody 1 x 120 uL 100X Biotinylated CTNNB1 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Catenin Beta 1 (CTNNb1) . No significant cross-reactivity or interference between Catenin Beta 1 (CTNNb1) and analogues was observed.
Protein Length
781
NCBI Gene Symbol
Ctnnb1
Protein Name
Catenin beta-1
Gene Name URL
Ctnnb1
Nucleotide Accession Number
NM_001165902.1

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