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CHGA ELISA Kit (Mouse)

CAT: 0247-OKCD02448-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02448-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Chromogranin A (CHGA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Chromogranin A (CHGA) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Chromogranin A
Gene Aliases
CgA; Ch; ChrA; chromogranin-A.
Gene ID
12652
Accession Number
NP_031719.1
Reactivity
Mouse|Mus musculus
Target
Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chromogranin A (CHGA) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chromogranin A (CHGA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chromogranin A (CHGA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chromogranin A (CHGA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
125-8,000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 89-101 93 EDTA Plasma (n=5) 90-98 95 Heparin Plasma (n=5) 79-93 87 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 89-101 93 Serum (n=5) 89-101 93 EDTA Plasma (n=5) 90-98 95 EDTA Plasma (n=5) 90-98 95 Heparin Plasma (n=5) 79-93 87 Heparin Plasma (n=5) 79-93 87
Sensitivity
48 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-CHGA Microplate 96 Wells (12 x 8 Well strips) CHGA Lyophilized Standard 2 100X Biotinylated CHGA Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-CHGA Microplate 96 Wells (12 x 8 Well strips) Anti-CHGA Microplate 96 Wells (12 x 8 Well strips) CHGA Lyophilized Standard 2 CHGA Lyophilized Standard 2 100X Biotinylated CHGA Detector Antibody 1 x 120 uL 100X Biotinylated CHGA Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Chromogranin A (CHGA) . No significant cross-reactivity or interference between Chromogranin A (CHGA) and analogues was observed.
Protein Length
463
NCBI Gene Symbol
Chga
Protein Name
Chromogranin-A
Gene Name URL
Chga
Nucleotide Accession Number
NM_007693.2

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