CEBPB ELISA Kit (Mouse)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level CCAAT/Enhancer Binding Protein Beta (CEBPb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level CCAAT/Enhancer Binding Protein Beta (CEBPb) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  CCAAT/enhancer binding protein (C/EBP), beta

• Gene Aliases:

  AGP/EBP; C/EBP BETA; C/EBPbe; C/EBPbeta; CCAAT/enhancer-binding protein beta; CR; CRP2; IL-6; IL-6DBP; interleukin-6-dependent-binding protein; LAP; LIP; liver-enriched transcriptional activator; Nf; NF-; Nfil6; NF-IL6; NF-M; nuclear protein Il6.

• Gene ID:

  12608

• Accession Number:

  NP_001274667.1

• Reactivity:

  Mouse|Mus musculus

• Target:

  Important transcription factor regulating the expression of genes involved in immune and inflammatory responses (PubMed:16585579, PubMed:17911624, PubMed:18486321, PubMed:20111005) . Plays also a significant role in adipogenesis, as well as in the gluconeogenic pathway, liver regeneration, and hematopoiesis (PubMed:9727068, PubMed:10635333, PubMed:17301242, PubMed:17601773, PubMed:19478079, PubMed:24061474, PubMed:24216764) . The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Its functional capacity is governed by protein interactions and post-translational protein modifications. During early embryogenesis, plays essential and redundant functions with CEBPA (PubMed:15509779) . Has a promitotic effect on many cell types such as hepatocytes and adipocytes but has an antiproliferative effect on T-cells by repressing MYC expression, facilitating differentiation along the T-helper 2 lineage (PubMed:9727068, PubMed:10635333, PubMed:16585579) . Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase reaction and inflammation. Plays also a role in intracellular bacteria killing (PubMed:17911624) . During adipogenesis, is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which turn on the series of adipocyte genes that give rise to the adipocyte phenotype. The delayed transactivation of the CEBPA and PPARG genes by CEBPB appears necessary to allow mitotic clonal expansion and thereby progression of terminal differentiation (PubMed:15985551, PubMed:17301242, PubMed:17601773, PubMed:20194620) . Essential for female reproduction because of a critical role in ovarian follicle development (PubMed:9303532) . Restricts osteoclastogenesis (PubMed:19440205) . Together with NFE2L1; represses expression of DSPP during odontoblast differentiation (By similarity) .

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCAAT/Enhancer Binding Protein Beta (CEBPb) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CCAAT/Enhancer Binding Protein Beta (CEBPb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CCAAT/Enhancer Binding Protein Beta (CEBPb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CCAAT/Enhancer Binding Protein Beta (CEBPb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Tissue homogenates, cell lysates and other biological fluids

• Detection Range:

  78-5,000pg/mL

• Sensitivity:

  33 pg/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount Anti-CEBPB Microplate 96 Wells (12 x 8 Well strips) CEBPB Lyophilized Standard 2 100X Biotinylated CEBPB Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-CEBPB Microplate 96 Wells (12 x 8 Well strips) Anti-CEBPB Microplate 96 Wells (12 x 8 Well strips) CEBPB Lyophilized Standard 2 CEBPB Lyophilized Standard 2 100X Biotinylated CEBPB Detector Antibody 1 x 120 uL 100X Biotinylated CEBPB Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of CCAAT/Enhancer Binding Protein Beta (CEBPb) . No significant cross-reactivity or interference between CCAAT/Enhancer Binding Protein Beta (CEBPb) and analogues was observed.

• Protein Length:

  296

• NCBI Gene Symbol:

  Cebpb

• Protein Name:

  CCAAT/enhancer-binding protein beta

• Gene Name URL:

  Cebpb

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001287738.1