CDH13 ELISA Kit (Mouse)

CAT: 0247-OKCD02442-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD02442-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Cadherin, Heart (CDHH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Cadherin, Heart (CDHH) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Cadherin 13
Gene Aliases
4932416G01Rik; cadherin-13; Cdht; H-cadherin; heart cadherin; Tca; Tcad; T-cadh; T-cadherin; Truncated cadherin.
Gene ID
12554
Accession Number
NP_062681.2
Reactivity
Mouse|Mus musculus
Target
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin, Heart (CDHH) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cadherin, Heart (CDHH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin, Heart (CDHH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cadherin, Heart (CDHH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
62.50-4000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 84-91 88 EDTA Plasma (n=5) 79-105 81 Heparin Plasma (n=5) 98-105 101 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 84-91 88 Serum (n=5) 84-91 88 EDTA Plasma (n=5) 79-105 81 EDTA Plasma (n=5) 79-105 81 Heparin Plasma (n=5) 98-105 101 Heparin Plasma (n=5) 98-105 101
Sensitivity
23.5 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-CDH13 Microplate 96 Wells (12 x 8 Well strips) CDH13 Lyophilized Standard 2 100X Biotinylated CDH13 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-CDH13 Microplate 96 Wells (12 x 8 Well strips) Anti-CDH13 Microplate 96 Wells (12 x 8 Well strips) CDH13 Lyophilized Standard 2 CDH13 Lyophilized Standard 2 100X Biotinylated CDH13 Detector Antibody 1 x 120 uL 100X Biotinylated CDH13 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Cadherin, Heart (CDHH) . No significant cross-reactivity or interference between Cadherin, Heart (CDHH) and analogues was observed.
Protein Length
714
NCBI Gene Symbol
Cdh13
Protein Name
Cadherin-13
Gene Name URL
Cdh13
Nucleotide Accession Number
NM_019707.5

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