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DFFB ELISA Kit (Human)

CAT: 0247-OKCD02412-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD02412-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Caspase Activated DNase (CAD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Caspase Activated DNase (CAD) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
DNA fragmentation factor subunit beta
Gene Aliases
CAD; caspase-activated deoxyribonuclease; caspase-activated DNase; caspase-activated nuclease; CPAN; DFF2; DFF40; DFF-40; DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) .
Gene ID
1677
Accession Number
NP_001307061.1
Reactivity
Homo sapiens|Human
Target
Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase Activated DNase (CAD) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase Activated DNase (CAD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase Activated DNase (CAD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Caspase Activated DNase (CAD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.052 ng/mL
Reconstitution
2Ā°C to 8Ā°C|-20Ā°C
Components
Component Amount Anti-DFFB Microplate 96 Wells (12 x 8 Well strips) DFFB Lyophilized Standard 2 100X Biotinylated DFFB Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-DFFB Microplate 96 Wells (12 x 8 Well strips) Anti-DFFB Microplate 96 Wells (12 x 8 Well strips) DFFB Lyophilized Standard 2 DFFB Lyophilized Standard 2 100X Biotinylated DFFB Detector Antibody 1 x 120 uL 100X Biotinylated DFFB Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Caspase Activated DNase (CAD) . No significant cross-reactivity or interference between Caspase Activated DNase (CAD) and analogues was observed.
Protein Length
338
NCBI Gene Symbol
DFFB
Protein Name
DNA fragmentation factor subunit beta
Gene Name URL
DFFB
Nucleotide Accession Number
NM_001320132.1

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