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DUOX2 ELISA Kit (Rat)

CAT: 0247-OKCD02397-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02397-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Dual Oxidase 2 (DUOX2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Dual Oxidase 2 (DUOX2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Dual oxidase 2
Gene Aliases
Dual oxidase 2; large NOX 2; long NOX 2; NADH/NADPH thyroid oxidase THOX2; Thox2; thyroid oxidase 2.
Gene ID
79107
Accession Number
NP_077055.1
Reactivity
Rat|Rattus norvegicus
Target
Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Oxidase 2 (DUOX2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Oxidase 2 (DUOX2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Oxidase 2 (DUOX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dual Oxidase 2 (DUOX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 90-98 95 EDTA Plasma (n=5) 78-97 88 Heparin Plasma (n=5) 80-94 83 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 90-98 95 Serum (n=5) 90-98 95 EDTA Plasma (n=5) 78-97 88 EDTA Plasma (n=5) 78-97 88 Heparin Plasma (n=5) 80-94 83 Heparin Plasma (n=5) 80-94 83
Sensitivity
0.063 ng/mL.
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-DUOX2 Microplate 96 Wells (12 x 8 Well strips) DUOX2 Lyophilized Standard 2 100X Biotinylated DUOX2 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-DUOX2 Microplate 96 Wells (12 x 8 Well strips) Anti-DUOX2 Microplate 96 Wells (12 x 8 Well strips) DUOX2 Lyophilized Standard 2 DUOX2 Lyophilized Standard 2 100X Biotinylated DUOX2 Detector Antibody 1 x 120 uL 100X Biotinylated DUOX2 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Dual Oxidase 2 (DUOX2) . No significant cross-reactivity or interference between Dual Oxidase 2 (DUOX2) and analogues was observed.
Protein Length
1517
NCBI Gene Symbol
Duox2
Protein Name
Dual oxidase 2
Gene Name URL
Duox2
Nucleotide Accession Number
NM_024141.1

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