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ASPN ELISA Kit (Mouse)

CAT: 0247-OKCD02387-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02387-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Asporin (ASPN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Asporin (ASPN) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Asporin
Gene Aliases
4631401G09Rik; AA986886; asporin; periodontal ligament-associated protein 1; PL; Plap1; SL; Slrr1c.
Gene ID
66695
Accession Number
NP_001165952.1
Reactivity
Mouse|Mus musculus
Target
Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity (By similarity) . Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins. Inhibits the interaction between TGFB1 and TGF-beta receptor type II in the presence of heparin/heparan sulfate in vitro.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Asporin (ASPN) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Asporin (ASPN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Asporin (ASPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Asporin (ASPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
15.6-1,000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 79-92 84 EDTA Plasma (n=5) 96-105 101 Heparin Plasma (n=5) 87-95 90 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 79-92 84 Serum (n=5) 79-92 84 EDTA Plasma (n=5) 96-105 101 EDTA Plasma (n=5) 96-105 101 Heparin Plasma (n=5) 87-95 90 Heparin Plasma (n=5) 87-95 90
Sensitivity
0.059 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-ASPN Microplate 96 Wells (12 x 8 Well strips) ASPN Lyophilized Standard 2 100X Biotinylated ASPN Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-ASPN Microplate 96 Wells (12 x 8 Well strips) Anti-ASPN Microplate 96 Wells (12 x 8 Well strips) ASPN Lyophilized Standard 2 ASPN Lyophilized Standard 2 100X Biotinylated ASPN Detector Antibody 1 x 120 uL 100X Biotinylated ASPN Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Asporin (ASPN) . No significant cross-reactivity or interference between Asporin (ASPN) and analogues was observed.
Protein Length
373
NCBI Gene Symbol
Aspn
Protein Name
Asporin
Gene Name URL
Aspn
Nucleotide Accession Number
NM_001172481.1

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