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ANXA5 ELISA Kit (Mouse)

CAT: 0247-OKCD02371-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02371-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Annexin V (ANXA5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Annexin V (ANXA5) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Annexin A5
Gene Aliases
An; anchorin CII; annexin A5; annexin V; annexin-5; Anx5; calphobindin I; CBP-I; endonexin II; lipocortin V; PAP-I; placental anticoagulant protein 4; placental anticoagulant protein I; PP4; R74653; thromboplastin inhibitor; VAC-alpha; vascular anticoagulant-alpha.
Gene ID
11747
Accession Number
NP_033803.1
Reactivity
Mouse|Mus musculus
Target
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Annexin V (ANXA5) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Annexin V (ANXA5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Annexin V (ANXA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Annexin V (ANXA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 82-89 85 EDTA Plasma (n=5) 83-104 93 Heparin Plasma (n=5) 89-97 94 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 82-89 85 Serum (n=5) 82-89 85 EDTA Plasma (n=5) 83-104 93 EDTA Plasma (n=5) 83-104 93 Heparin Plasma (n=5) 89-97 94 Heparin Plasma (n=5) 89-97 94
Sensitivity
6.4 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-ANXA5 Microplate 96 Wells (12 x 8 Well strips) ANXA5 Lyophilized Standard 2 100X Biotinylated ANXA5 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-ANXA5 Microplate 96 Wells (12 x 8 Well strips) Anti-ANXA5 Microplate 96 Wells (12 x 8 Well strips) ANXA5 Lyophilized Standard 2 ANXA5 Lyophilized Standard 2 100X Biotinylated ANXA5 Detector Antibody 1 x 120 uL 100X Biotinylated ANXA5 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Annexin A5 (ANXA5) . No significant cross-reactivity or interference between Annexin A5 (ANXA5) and analogues was observed.
Protein Length
319
NCBI Gene Symbol
Anxa5
Protein Name
Annexin A5
Gene Name URL
Anxa5
Nucleotide Accession Number
NM_009673.2

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