CD38 ELISA Kit (Rat)

CAT: 0247-OKCD02319-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD02319-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Cluster Of Differentiation 38 (CD38) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Cluster Of Differentiation 38 (CD38) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
CD38 molecule
Gene Aliases
2'-phospho-ADP-ribosyl cyclase;2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase;2'-phospho-cyclic-ADP-ribose transferase; ADPRC 1; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; CD38 antigen (ADP-ribosyl cyclase / cyclic ADP-ribose hydrolase) ; CD38H; cyclic ADP-ribose hydrolase 1.
Gene ID
25668
Accession Number
NP_037259.1
Reactivity
Rat|Rattus norvegicus
Target
Synthesizes the second messagers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 38 (CD38) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 38 (CD38) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 38 (CD38), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster Of Differentiation 38 (CD38) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
3.12-200ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 97-104 101 EDTA Plasma (n=5) 95-104 101 Heparin Plasma (n=5) 80-96 88 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 97-104 101 Serum (n=5) 97-104 101 EDTA Plasma (n=5) 95-104 101 EDTA Plasma (n=5) 95-104 101 Heparin Plasma (n=5) 80-96 88 Heparin Plasma (n=5) 80-96 88
Sensitivity
1.24 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-CD38 Microplate 96 Wells (12 x 8 Well strips) CD38 Lyophilized Standard 2 100X Biotinylated CD38 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-CD38 Microplate 96 Wells (12 x 8 Well strips) Anti-CD38 Microplate 96 Wells (12 x 8 Well strips) CD38 Lyophilized Standard 2 CD38 Lyophilized Standard 2 100X Biotinylated CD38 Detector Antibody 1 x 120 uL 100X Biotinylated CD38 Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Cyclic ADP Ribose Hydrolase (cADPRH) . No significant cross-reactivity or interference between Cyclic ADP Ribose Hydrolase (cADPRH) and analogues was observed.
Protein Length
303
NCBI Gene Symbol
Cd38
Protein Name
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
Gene Name URL
Cd38
Nucleotide Accession Number
NM_013127.1

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