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Beta-Endorphin ELISA Kit (Rat)

CAT: 0247-OKCD02294-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02294-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Aliases
Corticotropin-lipotropin.
Reactivity
Rat|Rattus norvegicus
Target
Corticotropin: Stimulates the adrenal glands to release cortisol.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Detection Method
Colorimetric
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Beta-Endorphin (bEP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Beta-Endorphin (bEP) and unlabeled Beta-Endorphin (bEP) (Standards or samples) with the pre-coated antibody specific to Beta-Endorphin (bEP) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids
Detection Range
12.35-1,000pg/mL
Recovery
Matrix Recovery Range (%) Average (%) serum (n=5) 80-97 93 EDTA plasma (n=5) 82-91 87 heparin plasma (n=5) 85-105 88 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 80-97 93 serum (n=5) 80-97 93 EDTA plasma (n=5) 82-91 87 EDTA plasma (n=5) 82-91 87 heparin plasma (n=5) 85-105 88 heparin plasma (n=5) 85-105 88
Sensitivity
4.62 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-Beta-Endorphin Microplate 96 Wells (12 x 8 Well Strips) Beta-Endorphin Lyophilized Standard 2 Beta-Endorphin Biotin Complex 1 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Biotin Complex Reconstitution Buffer 1 x 300 uL Component Amount Component Amount Anti-Beta-Endorphin Microplate 96 Wells (12 x 8 Well Strips) Anti-Beta-Endorphin Microplate 96 Wells (12 x 8 Well Strips) Beta-Endorphin Lyophilized Standard 2 Beta-Endorphin Lyophilized Standard 2 Beta-Endorphin Biotin Complex 1 Beta-Endorphin Biotin Complex 1 100X Avidin/HRP Conjugate 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL Biotin Complex Reconstitution Buffer 1 x 300 uL Biotin Complex Reconstitution Buffer 1 x 300 uL
NCBI Gene Symbol
BEP
Protein Name
Pro-opiomelanocortin
Gene Name URL
BEP

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