Lactic Acid ELISA Kit

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Lactic Acid (LA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Lactic Acid (LA) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

7732-18-5

Lactic acid; 2-hydroxypropanoic acid; DL-Lactic acid; 2-hydroxypropionic acid

All Species

Lactic acid is a colorless to yellow odorless syrupy liquid. Corrosive to metals and tissue. Used to make cultured dairy products, as a food preservative, and to make chemicals.

ELISA Kit

Enzyme-linked Immunosorbent assay-Competitive

Colorimetric

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Lactic Acid (LA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Lactic Acid (LA) and unlabeled Lactic Acid (LA) (Standards or samples) with the pre-coated antibody specific to Lactic Acid (LA) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Lactic Acid (LA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Lactic Acid (LA) in the sample.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, plasma and other biological fluids.

7.41-600ug/mL

Matrix Recovery Range (%) Average (%) _x005F serum (n=5) 89-101 97 _x005F EDTA plasma (n=5) 93-102 97 _x005F heparin plasma (n=5) 85-101 89 _x005F Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 89-101 97 serum (n=5) 89-101 97 EDTA plasma (n=5) 93-102 97 EDTA plasma (n=5) 93-102 97 heparin plasma (n=5) 85-101 89 heparin plasma (n=5) 85-101 89

2.92 ug/mL

2°C to 8°C|-20°C

Component Amount _x005F Anti-Lactic Acid Microplate 96 Wells (12 x 8 Well Strips) _x005F Lactic Acid Lyophilized Standard 2 _x005F Lactic Acid Biotin Complex 1 _x005F 100X Avidin/HRP Conjugate 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Biotin Complex Diluent 1 x 12 mL _x005F Avidin/HRP Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Biotin Complex Reconstitution Buffer 1 x 300 uL _x005F Component Amount Component Amount Anti-Lactic Acid Microplate 96 Wells (12 x 8 Well Strips) Anti-Lactic Acid Microplate 96 Wells (12 x 8 Well Strips) Lactic Acid Lyophilized Standard 2 Lactic Acid Lyophilized Standard 2 Lactic Acid Biotin Complex 1 Lactic Acid Biotin Complex 1 100X Avidin/HRP Conjugate 120 uL 100X Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Biotin Complex Diluent 1 x 12 mL Biotin Complex Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL Avidin/HRP Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL Biotin Complex Reconstitution Buffer 1 x 300 uL Biotin Complex Reconstitution Buffer 1 x 300 uL

LA

Lactic Acid