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H1F0 ELISA Kit (Human)

CAT: 0247-OKCD02090-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02090-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level H1 Histone Family, Member 0 (H1F0) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level H1 Histone Family, Member 0 (H1F0) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
H1.0 linker histone
Gene Aliases
H1 histone family member 0; H1.0; H1.0, H1 (0), H1-0; H10; H1F0; H1FV; histone H1'; histone H1 (0) ; histone H1.0.
Gene ID
3005
Accession Number
NP_005309.1
Reactivity
Homo sapiens|Human
Target
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to H1 Histone Family, Member 0 (H1F0) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to H1 Histone Family, Member 0 (H1F0) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain H1 Histone Family, Member 0 (H1F0), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of H1 Histone Family, Member 0 (H1F0) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.78-50ng/mL
Sensitivity
< 0.28 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-H1F0 Microplate 96 Wells (12 x 8 Well Strips) H1F0 Lyophilized Standard 2 x 50 ng 100X Biotinylated H1F0 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-H1F0 Microplate 96 Wells (12 x 8 Well Strips) Anti-H1F0 Microplate 96 Wells (12 x 8 Well Strips) H1F0 Lyophilized Standard 2 x 50 ng H1F0 Lyophilized Standard 2 x 50 ng 100X Biotinylated H1F0 Detector Antibody 120 uL 100X Biotinylated H1F0 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBZN2
Specificity
This assay has high sensitivity and excellent specificity for detection of H1 Histone Family, Member 0 (H1F0) . No significant cross-reactivity or interference between H1 Histone Family, Member 0 (H1F0) and analogues was observed.
Protein Length
194
NCBI Gene Symbol
H1-0
Protein Name
Histone H1.0
Gene Name URL
H1-0
Nucleotide Accession Number
NM_005318.3

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