SDPR ELISA Kit (Human)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Serum Deprivation Response Protein (SDPR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Serum Deprivation Response Protein (SDPR) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  Caveolae associated protein 2

• Gene Aliases:

  Caveolae-associated protein 2; cavin-2; phosphatidylserine-binding protein; PS-p68; SDPR; SDR; serum deprivation response; serum deprivation-response protein.

• Gene ID:

  8436

• Accession Number:

  NP_004648.1

• Reactivity:

  Homo sapiens|Human

• Target:

  Plays an important role in caveolar biogenesis and morphology. Regulates caveolae morphology by inducing membrane curvature within caveolae (PubMed:19525939) . Plays a role in caveola formation in a tissue-specific manner. Required for the formation of caveolae in the lung and fat endothelia but not in the heart endothelia. Negatively regulates the size or stability of CAVIN complexes in the lung endothelial cells. May play a role in targeting PRKCA to caveolae (By similarity) .

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serum Deprivation Response Protein (SDPR) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serum Deprivation Response Protein (SDPR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serum Deprivation Response Protein (SDPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Serum Deprivation Response Protein (SDPR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Tissue homogenates, cell lysates and other biological fluids

• Detection Range:

  0.156-10ng/mL

• Sensitivity:

  < 0.054 ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount _x005F Anti-SDPR Microplate 96 Wells (12 x 8 Well Strips) _x005F SDPR Lyophilized Standard 2 x 10 ng _x005F 100X Biotinylated SDPR Detector Antibody 120 uL _x005F Avidin/HRP Conjugate 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Component Amount Component Amount Anti-SDPR Microplate 96 Wells (12 x 8 Well Strips) Anti-SDPR Microplate 96 Wells (12 x 8 Well Strips) SDPR Lyophilized Standard 2 x 10 ng SDPR Lyophilized Standard 2 x 10 ng 100X Biotinylated SDPR Detector Antibody 120 uL 100X Biotinylated SDPR Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes:

  Formerly GWB-KBBUC7

• Specificity:

  This assay has high sensitivity and excellent specificity for detection of Serum Deprivation Response Protein (SDPR) ._x005F No significant cross-reactivity or interference between Serum Deprivation Response Protein (SDPR) and analogues was observed.

• Protein Length:

  425

• NCBI Gene Symbol:

  CAVIN2

• Protein Name:

  Caveolae-associated protein 2

• Gene Name URL:

  CAVIN2

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_004657.5