SSH2 ELISA Kit (Human)

CAT: 0247-OKCD02002-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD02002-96WSize:96 Well
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Slingshot Homolog 2 (SSH2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Slingshot Homolog 2 (SSH2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Slingshot protein phosphatase 2
Gene Aliases
Protein phosphatase Slingshot homolog 2; SSH-2; SSH-2L; SSH-like protein 2.
Gene ID
85464
Accession Number
NP_001269058.1
Reactivity
Homo sapiens|Human
Target
Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Slingshot Homolog 2 (SSH2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Slingshot Homolog 2 (SSH2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Slingshot Homolog 2 (SSH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Slingshot Homolog 2 (SSH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.113 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-CILP2 Microplate 96 Wells (12 x 8 Well Strips) CILP2 Lyophilized Standard 2 x 20 ng 100X Biotinylated CILP2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 7 mL Component Amount Component Amount Anti-CILP2 Microplate 96 Wells (12 x 8 Well Strips) Anti-CILP2 Microplate 96 Wells (12 x 8 Well Strips) CILP2 Lyophilized Standard 2 x 20 ng CILP2 Lyophilized Standard 2 x 20 ng 100X Biotinylated CILP2 Detector Antibody 120 uL 100X Biotinylated CILP2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 7 mL Stop Solution 1 x 7 mL
Notes
Formerly GWB-KBBJF5
Specificity
This assay has high sensitivity and excellent specificity for detection of Slingshot Homolog 2 (SSH2) . No significant cross-reactivity or interference between Slingshot Homolog 2 (SSH2) and analogues was observed.
Protein Length
1423
NCBI Gene Symbol
SSH2
Protein Name
Protein phosphatase Slingshot homolog 2
Gene Name URL
SSH2
Nucleotide Accession Number
NM_001282129.1

Related Products

CatalogName
OAAN04089-50ULSSH2 Antibody
OAAN04089-200ULSSH2 Antibody
OAAN04089-100ULSSH2 Antibody
OKCD02003SSH1 ELISA Kit (Human) (OKCD02003)
OKCD09199SSH3 ELISA Kit (Human) (OKCD09199)
OKDD00544SSH3 ELISA Kit (Human) (OKDD00544)
OKCD09199-96WOKCD09199-96W - SSH3 ELISA Kit (Human)
OKCD02003-96WOKCD02003-96W - SSH1 ELISA Kit (Human)
OKCD06003-96WKIT ELISA Kit (Human)
OKEH00203-96WKIT ELISA Kit (Human)

Alternative Products

CatalogName
E035404Human SSH2 ELISA Kit
MBS2706694-01Human Slingshot Homolog 2 (SSH2) ELISA Kit
MBS8803410-01Human SSH2 (Slingshot Homolog 2) ELISA Kit
H1084Slingshot Homolog 2 (SSH2) Human ELISA Kit
KBH22514GENLISA™ Human Slingshot Homolog 2 (SSH2) ELISA
028100Slingshot Homolog 2 (SSH2) BioAssayTM ELISA Kit (Human)
JL17560-01Human Protein Phosphatase Slingshot Homolog 2 (SSH2) ELISA Kit
G3946Protein Phosphatase Slingshot homolog 2 (SSH2) Human ELISA Kit
KTE60367-01Human Protein phosphatase Slingshot homolog 2 (SSH2) ELISA Kit
MBS8200539-01SSH2 siRNA (Human)

Popular Products