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DGAT2 ELISA Kit (Human)

CAT: 0247-OKCD01987-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01987-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Diacylglycerol O-acyltransferase 2
Gene Aliases
Acyl-CoA retinol O-fatty-acyltransferase; ARAT; diacylglycerol O-acyltransferase 2; diacylglycerol O-acyltransferase homolog 2; diacylglycerol O-acyltransferase-like protein 2; diglyceride acyltransferase 2; GS1999FULL; HMFN1045.
Gene ID
84649
Accession Number
NP_001240820.1
Reactivity
Homo sapiens|Human
Target
Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides (By similarity) . Functions also as an acyl-CoA retinol acyltransferase (ARAT) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
3.12-200ng/mL
Sensitivity
< 1.33 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-DGAT2 Microplate 96 Wells (12 x 8 Well Strips) DGAT2 Lyophilized Standard 2 x 200 ng 100X Biotinylated DGAT2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-DGAT2 Microplate 96 Wells (12 x 8 Well Strips) Anti-DGAT2 Microplate 96 Wells (12 x 8 Well Strips) DGAT2 Lyophilized Standard 2 x 200 ng DGAT2 Lyophilized Standard 2 x 200 ng 100X Biotinylated DGAT2 Detector Antibody 120 uL 100X Biotinylated DGAT2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBOZ8
Specificity
This assay has high sensitivity and excellent specificity for detection of Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2) . No significant cross-reactivity or interference between Diacylglycerol-O-Acyltransferase Homolog 2 (DGAT2) and analogues was observed.
Protein Length
388
NCBI Gene Symbol
DGAT2
Protein Name
Diacylglycerol O-acyltransferase 2
Gene Name URL
DGAT2
Nucleotide Accession Number
NM_001253891.1

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