TRPM8 ELISA Kit (Human)

CAT: 0247-OKCD01884-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD01884-96WSize:96 Well
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Transient receptor potential cation channel subfamily M member 8
Gene Aliases
Long transient receptor potential channel 6; LTrpC-6; LTRPC6; transient receptor potential cation channel subfamily M member 8; transient receptor potential p8; transient receptor potential subfamily M member 8; TRPM8 cationic channel; trp-p8; TRPP8.
Gene ID
79054
Accession Number
NP_076985.4
Reactivity
Homo sapiens|Human
Target
Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonist menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. In prostate cancer cells, shows strong inward rectification and high calcium selectivity in contrast to its behavior in normal cells which is characterized by outward rectification and poor cationic selectivity. Plays a role in prostate cancer cell migration (PubMed:25559186) . Isoform 2 and isoform 3 negatively regulate menthol- and cold-induced channel activity by stabilizing the closed state of the channel.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Matrix Recovery Range (%) Average (%) Serum 91-98 94 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Serum 91-98 94 Serum 91-98 94
Sensitivity
< 0.112 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-TRPM8 Microplate 96 Wells (12 x 8 Well Strips) TRPM8 Lyophilized Standard 2 x 20 ng 100X Biotinylated TRPM8 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-TRPM8 Microplate 96 Wells (12 x 8 Well Strips) Anti-TRPM8 Microplate 96 Wells (12 x 8 Well Strips) TRPM8 Lyophilized Standard 2 x 20 ng TRPM8 Lyophilized Standard 2 x 20 ng 100X Biotinylated TRPM8 Detector Antibody 120 uL 100X Biotinylated TRPM8 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBVE9
Specificity
This assay has high sensitivity and excellent specificity for detection of Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8) . No significant cross-reactivity or interference between Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8) and analogues was observed.
Protein Length
1104
NCBI Gene Symbol
TRPM8
Protein Name
Transient receptor potential cation channel subfamily M member 8
Gene Name URL
TRPM8
Nucleotide Accession Number
NM_024080.4

Related Products

CatalogName
OACA05177-50UGTRPM8 Antibody
OAAB05079-400ULTRPM8 Antibody
OACA07796-100ULTRPM8 Antibody
OACA05177-100UGTRPM8 Antibody
OACA07796-50ULTRPM8 Antibody
OCOA02701-20UGTRPM8 Protein Lysate
OAAB08012-400ULTRPM8 Antibody - middle region
OKEH07814-96WTRPM7 ELISA Kit (Human)
ARP35357_P050-25ULTRPM8 Antibody - N-terminal region
OAAB05080-400ULTRPM8 Antibody - C-terminal region

Alternative Products

CatalogName
EF003869Human TRPM8 ELISA KIT
MBS8803958-01Human TRPM8 (Transient Receptor Potential Cation Channel Subfamily M, Member 8) ELISA Kit
MBS9328644-01Human Transient receptor potential Cation channel subfamily M member 8, TRPM8 ELISA Kit
MBS2024711-01Human Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8) ELISA Kit
MBS7607003-01Human TRPM8 (Transient receptor potential cation channel subfamily M member 8) ELISA Kit
KTE60129-01Human Transient receptor potential cation channel subfamily M member 8 (TRPM8) ELISA Kit
JOT-EK3735HuHuman Transient receptor potential cation channel subfamily M member 8,TRPM8 ELISA KIT
H7775Transient Receptor potential cation channel subfamily M member 8 (TRPM8) Human ELISA Kit
RK02451Human Transient Receptor Potential Cation Channel Subfamily M, Member 8 ELISA Kit (TRPM8)
KBH3735GENLISA™ Human Transient Receptor Potential Cation Channel Subfamily M (Member 8 (TRPM8) ELISA

Popular Products