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TMPRSS6 ELISA Kit (Human)

CAT: 0247-OKCD01883-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01883-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Transmembrane Protease, Serine 6 (TMPRSS6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Transmembrane Protease, Serine 6 (TMPRSS6) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Transmembrane serine protease 6
Gene Aliases
IRIDA; matriptase-2; membrane-bound mosaic serine proteinase matriptase-2; MT2; transmembrane protease serine 6; transmembrane protease, serine 6; type II transmembrane serine protease 6.
Gene ID
164656
Accession Number
NP_001275929.1
Reactivity
Homo sapiens|Human
Target
Serine protease which hydrolyzes a range of proteins including type I collagen, fibronectin and fibrinogen. Can also activate urokinase-type plasminogen activator with low efficiency. May play a specialized role in matrix remodeling processes in liver. Through the cleavage of HFE2, a regulator of the expression of the iron absorption-regulating hormone hepicidin/HAMP, plays a role in iron homeostasis.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane Protease, Serine 6 (TMPRSS6) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transmembrane Protease, Serine 6 (TMPRSS6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transmembrane Protease, Serine 6 (TMPRSS6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transmembrane Protease, Serine 6 (TMPRSS6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.78-50ng/mL
Sensitivity
< 0.27 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-TMPRSS6 Microplate 96 Wells (12 x 8 Well Strips) TMPRSS6 Lyophilized Standard 2 x 50 ng 100X Biotinylated TMPRSS6 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-TMPRSS6 Microplate 96 Wells (12 x 8 Well Strips) Anti-TMPRSS6 Microplate 96 Wells (12 x 8 Well Strips) TMPRSS6 Lyophilized Standard 2 x 50 ng TMPRSS6 Lyophilized Standard 2 x 50 ng 100X Biotinylated TMPRSS6 Detector Antibody 120 uL 100X Biotinylated TMPRSS6 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBHD2
Specificity
This assay has high sensitivity and excellent specificity for detection of Transmembrane Protease, Serine 6 (TMPRSS6) . No significant cross-reactivity or interference between Transmembrane Protease, Serine 6 (TMPRSS6) and analogues was observed.
Protein Length
811
NCBI Gene Symbol
TMPRSS6
Protein Name
Transmembrane protease serine 6
Gene Name URL
TMPRSS6
Nucleotide Accession Number
NM_001289000.1

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