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Cacna1e ELISA Kit (Mouse)

CAT: 0247-OKCD01859-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01859-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Calcium channel, voltage-dependent, R type, alpha 1E subunit
Gene Aliases
Brain calcium channel II; Calcium channel, L type, alpha-1 polypeptide, isoform 6; Voltage-gated calcium channel subunit alpha Cav2.3.
Gene ID
12290
Accession Number
NP_033912.2
Reactivity
Mouse|Mus musculus
Target
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1E gives rise to R-type calcium currents. R-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by nickel, and partially by omega-agatoxin-IIIA (omega-Aga-IIIA) . They are however insensitive to dihydropyridines (DHP), omega-conotoxin-GVIA (omega-CTx-GVIA), and omega-agatoxin-IVA (omega-Aga-IVA) . Calcium channels containing alpha-1E subunit could be involved in the modulation of firing patterns of neurons which is important for information processing.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.125 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-Cacna1e Microplate 96 Wells (12 x 8 Well Strips) Cacna1e Lyophilized Standard 2 x 20 ng 100X Biotinylated Cacna1e Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Cacna1e Microplate 96 Wells (12 x 8 Well Strips) Anti-Cacna1e Microplate 96 Wells (12 x 8 Well Strips) Cacna1e Lyophilized Standard 2 x 20 ng Cacna1e Lyophilized Standard 2 x 20 ng 100X Biotinylated Cacna1e Detector Antibody 120 uL 100X Biotinylated Cacna1e Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBAK4
Specificity
This assay has high sensitivity and excellent specificity for detection of Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) . No significant cross-reactivity or interference between Calcium Channel, Voltage Dependent, R-Type, Alpha 1E Subunit (CACNa1E) and analogues was observed.
Protein Length
2272
NCBI Gene Symbol
CACNA1E
Protein Name
Voltage-dependent R-type calcium channel subunit alpha-1E
Gene Name URL
CACNA1E
Nucleotide Accession Number
NM_009782.3

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