WASF2 ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

7732-18-5

WASP family member 2

DJ393P12.2; IMD2; protein WAVE-2; putative Wiskott-Aldrich syndrome protein family member 4; SCAR2; suppressor of cyclic-AMP receptor (WASP-family) ; verprolin homology domain-containing protein 2; WAS protein family member 2; WASF4; WASP family protein member 2; WASP family protein member 4; WASP family Verprolin-homologous protein 2; WAVE2; wiskott-Aldrich syndrome protein family member 2.

10163

NP_001188333.1

Homo sapiens|Human

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Tissue homogenates and other biological fluids

0.312-20ng/mL

< 0.106 ng/mL

2°C to 8°C|-20°C

Component Amount Anti-WASF2 Microplate 96 Wells (12 x 8 Well Strips) WASF2 Lyophilized Standard 2 x 20 ng 100X Biotinylated WASF2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-WASF2 Microplate 96 Wells (12 x 8 Well Strips) Anti-WASF2 Microplate 96 Wells (12 x 8 Well Strips) WASF2 Lyophilized Standard 2 x 20 ng WASF2 Lyophilized Standard 2 x 20 ng 100X Biotinylated WASF2 Detector Antibody 120 uL 100X Biotinylated WASF2 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

Formerly GWB-KBBFT2

This assay has high sensitivity and excellent specificity for detection of Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) . No significant cross-reactivity or interference between Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) and analogues was observed.

498

WASF2

Wiskott-Aldrich syndrome protein family member 2

WASF2