Sort1 ELISA Kit (Mouse)

CAT: 0247-OKCD01711-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD01711-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Sortilin 1 (SORT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Sortilin 1 (SORT1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Sortilin 1
Gene Aliases
2900053A11Rik; AI852375; mNTR3; neurotensin receptor 3; Nt; Ntr3; Ntsr3; s; sortilin.
Gene ID
20661
Accession Number
NP_001258528.1
Reactivity
Mouse|Mus musculus
Target
Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR) . Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB) . Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs) . These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sortilin 1 (SORT1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sortilin 1 (SORT1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sortilin 1 (SORT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sortilin 1 (SORT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Matrix Recovery Range (%) Average (%) Serum 82-102 95 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Serum 82-102 95 Serum 82-102 95
Sensitivity
< 0.066 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-Sort1 Microplate 96 Wells (12 x 8 Well Strips) Sort1 Lyophilized Standard 2 x 10 ng 100X Biotinylated Sort1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Sort1 Microplate 96 Wells (12 x 8 Well Strips) Anti-Sort1 Microplate 96 Wells (12 x 8 Well Strips) Sort1 Lyophilized Standard 2 x 10 ng Sort1 Lyophilized Standard 2 x 10 ng 100X Biotinylated Sort1 Detector Antibody 120 uL 100X Biotinylated Sort1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBEX2
Specificity
This assay has high sensitivity and excellent specificity for detection of Sortilin 1 (SORT1) . No significant cross-reactivity or interference between Sortilin 1 (SORT1) and analogues was observed.
Protein Length
825
NCBI Gene Symbol
Sort1
Protein Name
Sortilin
Gene Name URL
Sort1
Nucleotide Accession Number
NM_001271599.1

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