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Ryr1 ELISA Kit (Rat)

CAT: 0247-OKCD01700-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01700-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Ryanodine Receptor 1, Skeletal (RYR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Ryanodine Receptor 1, Skeletal (RYR1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Ryanodine receptor 1
Gene Aliases
Skeletal muscle calcium release channel; Skeletal muscle ryanodine receptor; Skeletal muscle-type ryanodine receptor; Type 1 ryanodine receptor.
Gene ID
114207
Accession Number
XP_017445931.1
Reactivity
Rat|Rattus norvegicus
Target
Calcium channel that mediates the release of Ca (2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules (PubMed:11316255) . Repeated very high-level exercise increases the open probability of the channel and leads to Ca (2+) leaking into the cytoplasm (By similarity) . Can also mediate the release of Ca (2+) from intracellular stores in neurons, and may thereby promote prolonged Ca (2+) signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ryanodine Receptor 1, Skeletal (RYR1) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ryanodine Receptor 1, Skeletal (RYR1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ryanodine Receptor 1, Skeletal (RYR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ryanodine Receptor 1, Skeletal (RYR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.625-40ng/mL
Sensitivity
< 0.248 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-Ryr1 Microplate 96 Wells (12 x 8 Well Strips) Ryr1 Lyophilized Standard 2 x 40 ng 100X Biotinylated Ryr1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Ryr1 Microplate 96 Wells (12 x 8 Well Strips) Anti-Ryr1 Microplate 96 Wells (12 x 8 Well Strips) Ryr1 Lyophilized Standard 2 x 40 ng Ryr1 Lyophilized Standard 2 x 40 ng 100X Biotinylated Ryr1 Detector Antibody 120 uL 100X Biotinylated Ryr1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBAB4
Specificity
This assay has high sensitivity and excellent specificity for detection of Ryanodine Receptor 1, Skeletal (RYR1) . No significant cross-reactivity or interference between Ryanodine Receptor 1, Skeletal (RYR1) and analogues was observed.
Protein Length
5035
NCBI Gene Symbol
RYR1
Protein Name
Ryanodine receptor 1
Gene Name URL
RYR1
Nucleotide Accession Number
XM_017604710.1

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