Products for Research Use Only
RegisterLogin

IL6 ELISA Kit (Goat)

CAT: 0247-OKCD01461-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKCD01461-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Interleukin 6 (IL6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Interleukin 6 (IL6) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Interleukin 6
Gene Aliases
IL-6; interleukin 6 (interferon, beta 2) ; interleukin-6.
Gene ID
100860785
Accession Number
NP_001272569.1
Reactivity
Capra hircus|Goat
Target
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T (H) 17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL6) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 (IL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 6 (IL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.6-1,000pg/mL
Recovery
Matrix Recovery Range (%) Average (%) _x005F Serum (n=5) 95-103 98 _x005F EDTA Plasma (n=5) 80-102 83 _x005F Heparin Plasma (n=5) 83-91 87 _x005F Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Serum (n=5) 95-103 98 Serum (n=5) 95-103 98 EDTA Plasma (n=5) 80-102 83 EDTA Plasma (n=5) 80-102 83 Heparin Plasma (n=5) 83-91 87 Heparin Plasma (n=5) 83-91 87
Sensitivity
< 5.8 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount _x005F Anti-IL6 Microplate 96 Wells (12 x 8 Well Strips) _x005F IL6 Lyophilized Standard 2 x 1 ng _x005F 100X Biotinylated IL6 Detector Antibody 120 uL _x005F Avidin/HRP Conjugate 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Component Amount Component Amount Anti-IL6 Microplate 96 Wells (12 x 8 Well Strips) Anti-IL6 Microplate 96 Wells (12 x 8 Well Strips) IL6 Lyophilized Standard 2 x 1 ng IL6 Lyophilized Standard 2 x 1 ng 100X Biotinylated IL6 Detector Antibody 120 uL 100X Biotinylated IL6 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBAS8
Specificity
This assay has high sensitivity and excellent specificity for detection of Interleukin 6 (IL6) ._x005F No significant cross-reactivity or interference between Interleukin 6 (IL6) and analogues was observed.
Protein Length
208
NCBI Gene Symbol
IL6
Protein Name
Interleukin-6
Gene Name URL
IL6
Nucleotide Accession Number
NM_001285640.1

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number7732-18-5
PubChem CID962
IUPAC Nameoxidane
Molecular FormulaH2O
Molecular Weight18.015
XLogP-0.5
Topological Polar Surface Area1
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Heavy Atom Count1
Formal Charge0
Complexity0
SMILES
O
InChI
InChI=1S/H2O/h1H2
InChIKey
XLYOFNOQVPJJNP-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of Water
CAS: 7732-18-5
CID: 962
Formula: H2O
MW: 18.015
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight18.015
XLogP3-0.5
Hydrogen Bond Donor Count1
Hydrogen Bond Acceptor Count1
Rotatable Bond Count0
Exact Mass18.010564683
Monoisotopic Mass18.010564683
Topological Polar Surface Area1 Ų
Heavy Atom Count1
Formal Charge0
Complexity0
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

Related Products

CatalogName
OKCD01307-96WIL6 High Sensitivity ELISA Kit (Goat)
OKCD01307IL6 High Sensitivity ELISA Kit (Goat) (OKCD01307)
OKEH00140-96WIL6 ELISA Kit (Human)
OKCD00107-96WIL6 ELISA Kit (Monkey)
OKCD01463-96WIL6 ELISA Kit (Sheep)
OKEH00142-96WIL6 ELISA Kit (Mouse)
OKCD01462-96WIL6 ELISA Kit (Horse)
OKCD05863-96WIL6 ELISA Kit (Pig)
OKDD04123-96WIL6 ELISA Kit (Horse)
OKEH00141-96WIL6 ELISA Kit (Chicken)

Alternative Products

CatalogName
GTR10475235Goat Cholesterol ELISA ELISA
EGTA0893Goat AT ELISA Kit
EGTO0024Goat ON ELISA Kit
KT-804Goat IgA ELISA
GTR10411556Goat Microtransferrinuria ELISA
GTR10386374Goat Hydroxylysine ELISA
EGTN0048Goat NO ELISA Kit
GTR10415514Goat Alantoine ELISA
KT-802Goat IgM ELISA
0801223Goat IgG ELISA

Popular Products