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MBP High Sensitivity ELISA Kit (Human)

CAT: 0247-OKCD01323-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01323-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level High Sensitive Myelin Basic Protein (MBP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level High Sensitive Myelin Basic Protein (MBP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Myelin basic protein
Gene Aliases
Golli-MBP; myelin A1 protein; myelin basic protein; myelin membrane encephalitogenic protein; proline-rich proteoglycan 2.
Gene ID
4155
Accession Number
NP_001020252.1
Reactivity
Homo sapiens|Human
Target
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Myelin Basic Protein (MBP) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Myelin Basic Protein (MBP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Myelin Basic Protein (MBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Myelin Basic Protein (MBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, cerebrospinal fluid and other biological fluids
Detection Range
15.6-1,000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 95-104 101 EDTA Plasma (n=5) 79-91 82 Heparin Plasma (n=5) 90-104 98 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 95-104 101 Serum (n=5) 95-104 101 EDTA Plasma (n=5) 79-91 82 EDTA Plasma (n=5) 79-91 82 Heparin Plasma (n=5) 90-104 98 Heparin Plasma (n=5) 90-104 98
Sensitivity
6.4 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-MBP Microplate 96 Wells (12 x 8 Well Strips) MBP Lyophilized Standard 2 100X Biotinylated MBP Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-MBP Microplate 96 Wells (12 x 8 Well Strips) Anti-MBP Microplate 96 Wells (12 x 8 Well Strips) MBP Lyophilized Standard 2 MBP Lyophilized Standard 2 100X Biotinylated MBP Detector Antibody 120 uL 100X Biotinylated MBP Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Myelin Basic Protein (MBP) . No significant cross-reactivity or interference between Myelin Basic Protein (MBP) and analogues was observed.
NCBI Gene Symbol
MBP
Protein Name
Myelin basic protein
Gene Name URL
MBP
Nucleotide Accession Number
NM_001025081.1

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