C Reactive Protein Antibody ELISA Kit (Human)

• Reproducibility:

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Anti-C Reactive Protein Antibody (Anti-CRP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Anti-C Reactive Protein Antibody (Anti-CRP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• Gene Name:

  C-reactive protein

• Gene Aliases:

  C-reactive protein; C-reactive protein, pentraxin-related; pentraxin 1; PTX1.

• Gene ID:

  1401

• Reactivity:

  Homo sapiens|Human

• Target:

  Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Competitive

• Detection Method:

  Colorimetric

• Assay Principle:

  The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-C Reactive Protein Antibody (Anti-CRP) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-C Reactive Protein Antibody (Anti-CRP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  3.12-200ng/mL

• Recovery:

  Matrix Recovery range (%) Average (%) Serum (n=5) 97-105 101 EDTA Plasma (n=5) 81-91 85 Heparin Plasma (n=5) 82-95 90 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 97-105 101 Serum (n=5) 97-105 101 EDTA Plasma (n=5) 81-91 85 EDTA Plasma (n=5) 81-91 85 Heparin Plasma (n=5) 82-95 90 Heparin Plasma (n=5) 82-95 90

• Sensitivity:

  1.14 ng/mL

• Reconstitution:

  2°C to 8°C|-20°C

• Components:

  Component Amount C Reactive Protein antibody Microplate 96 Wells (12 x 8 Well strips) C Reactive Protein antibody Lyophilized Standard 2 x XXX 100X Detector Antibody 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount C Reactive Protein antibody Microplate 96 Wells (12 x 8 Well strips) C Reactive Protein antibody Microplate 96 Wells (12 x 8 Well strips) C Reactive Protein antibody Lyophilized Standard 2 x XXX C Reactive Protein antibody Lyophilized Standard 2 x XXX 100X Detector Antibody 1 x 120 uL 100X Detector Antibody 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL

• NCBI Gene Symbol:

  CRP

• Protein Name:

  C-reactive protein

• Gene Name URL:

  CRP

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_000567.2