SGCD ELISA Kit (Human)

CAT: 0247-OKCD01259-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD01259-96WSize:96 Well
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Sarcoglycan Delta (SGCd) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Sarcoglycan Delta (SGCd) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Sarcoglycan delta
Gene Aliases
35 kDa dystrophin-associated glycoprotein;35DAG; CMD1L; DAGD; delta-sarcoglycan; delta-SG; dystrophin associated glycoprotein, delta sarcoglycan; LGMDR6; placental delta sarcoglycan; sarcoglycan, delta (35kDa dystrophin-associated glycoprotein) ; SGCDP; SGD; SG-delta.
Gene ID
6444
Accession Number
NP_000328
Reactivity
Homo sapiens|Human
Target
Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sarcoglycan Delta (SGCd) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sarcoglycan Delta (SGCd) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sarcoglycan Delta (SGCd), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sarcoglycan Delta (SGCd) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.781-50ng/mL
Sensitivity
< 0.28 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-SGCD Microplate 96 Wells (12 x 8 Well Strips) SGCD Lyophilized Standard 2 x 50 ng 100X Biotinylated SGCD Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-SGCD Microplate 96 Wells (12 x 8 Well Strips) Anti-SGCD Microplate 96 Wells (12 x 8 Well Strips) SGCD Lyophilized Standard 2 x 50 ng SGCD Lyophilized Standard 2 x 50 ng 100X Biotinylated SGCD Detector Antibody 120 uL 100X Biotinylated SGCD Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBUI8
Specificity
This assay has high sensitivity and excellent specificity for detection of Sarcoglycan Delta (SGCd) . No significant cross-reactivity or interference between Sarcoglycan Delta (SGCd) and analogues was observed.
Protein Length
289
NCBI Gene Symbol
SGCD
Protein Name
Delta-sarcoglycan
Gene Name URL
SGCD
Nucleotide Accession Number
NM_000337.5

Related Products

CatalogName
OACA08211-50ULSGCD Antibody
OACD07251-100ULSGCD Antibody
OACA08211-100ULSGCD Antibody
OACD07251-1MLSGCD Antibody
OCOA18588-20UGSGCD Protein Lysate
OCOA05228-20UGSGCD Protein Lysate
OPCD06939-10UGSGCD Recombinant Protein
OCOA00119-20UGSGCD Protein Lysate
OPCD06939-200UGSGCD Recombinant Protein
OPCD06939-50UGSGCD Recombinant Protein

Alternative Products

CatalogName
E034231Human SGCd ELISA Kit
EH25715Human SGCd (Sarcoglycan Delta) ELISA Kit
MBS2018887-01Human Sarcoglycan Delta (SGCd) ELISA Kit
G8918Sarcoglycan Delta (SGCd) Human ELISA Kit
MBS2889876-01Human SGCD/Delta-Sarcoglycan ELISA Kit
C8546Delta-sarcoglycan (SGCD) Human ELISA Kit
EK7346Human Delta-sarcoglycan (SGCD) ELISA Kit
KBH22394GENLISA™ Human Sarcoglycan Delta (SGCd) ELISA
OM302235-01SGCD Human
MBS8240228-01SGCD siRNA (Human)

Popular Products