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WWC1 ELISA Kit (Mouse)

CAT: 0247-OKCD01222-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01222-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Kidney And Brain Protein (KIBRA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Kidney And Brain Protein (KIBRA) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
WW, C2 and coiled-coil domain containing 1
Gene Aliases
AA408228; AU017197; BC037006; Ki; Kibra; kidney and brain protein; protein KIBRA; protein WWC1; WW domain-containing protein 1.
Gene ID
211652
Accession Number
NP_740749
Reactivity
Mouse|Mus musculus
Target
Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with NF2 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. Acts as a transcriptional coactivator of ESR1 which plays an essential role in DYNLL1-mediated ESR1 transactivation. Regulates collagen-stimulated activation of the ERK/MAPK cascade. Modulates directional migration of podocytes. Acts as a substrate for PRKCZ and may be associated with memory performance (By similarity) . Regulates collagen-stimulated activation of the ERK/MAPK cascade.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kidney And Brain Protein (KIBRA) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kidney And Brain Protein (KIBRA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kidney And Brain Protein (KIBRA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kidney And Brain Protein (KIBRA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
78.12-5000pg/mL
Sensitivity
< 30 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-Wwc1 Microplate 96 Wells (12 x 8 Well Strips) Wwc1 Lyophilized Standard 2 x 5 ng 100X Biotinylated Wwc1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Wwc1 Microplate 96 Wells (12 x 8 Well Strips) Anti-Wwc1 Microplate 96 Wells (12 x 8 Well Strips) Wwc1 Lyophilized Standard 2 x 5 ng Wwc1 Lyophilized Standard 2 x 5 ng 100X Biotinylated Wwc1 Detector Antibody 120 uL 100X Biotinylated Wwc1 Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBCV2
Specificity
This assay has high sensitivity and excellent specificity for detection of Kidney And Brain Protein (KIBRA) . No significant cross-reactivity or interference between Kidney And Brain Protein (KIBRA) and analogues was observed.
Protein Length
1104
NCBI Gene Symbol
Wwc1
Protein Name
Protein KIBRA
Gene Name URL
Wwc1
Nucleotide Accession Number
NM_170779.1

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