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CACYBP ELISA Kit (Human)

CAT: 0247-OKCD01203-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01203-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Calcyclin Binding Protein (CACYBP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Calcyclin Binding Protein (CACYBP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Calcyclin binding protein
Gene Aliases
Calcyclin-binding protein; GIG5; growth-inhibiting gene 5 protein; hCacyBP; PNAS-107; S100A6-binding protein; S100A6BP; Siah-interacting protein; Siah-interacting protein (SIP) ; SIP.
Gene ID
27101
Accession Number
NP_001007215
Reactivity
Homo sapiens|Human
Target
May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcyclin Binding Protein (CACYBP) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcyclin Binding Protein (CACYBP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcyclin Binding Protein (CACYBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calcyclin Binding Protein (CACYBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
78.12-5000pg/mL
Sensitivity
< 33 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-CACYBP Microplate 96 Wells (12 x 8 Well Strips) CACYBP Lyophilized Standard 2 x 5 ng 100X Biotinylated CACYBP Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-CACYBP Microplate 96 Wells (12 x 8 Well Strips) Anti-CACYBP Microplate 96 Wells (12 x 8 Well Strips) CACYBP Lyophilized Standard 2 x 5 ng CACYBP Lyophilized Standard 2 x 5 ng 100X Biotinylated CACYBP Detector Antibody 120 uL 100X Biotinylated CACYBP Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBXD1
Specificity
This assay has high sensitivity and excellent specificity for detection of Calcyclin Binding Protein (CACYBP) . No significant cross-reactivity or interference between Calcyclin Binding Protein (CACYBP) and analogues was observed.
Protein Length
228
NCBI Gene Symbol
CACYBP
Protein Name
Calcyclin-binding protein
Gene Name URL
CACYBP
Nucleotide Accession Number
NM_001007214.1

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