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FDPS ELISA Kit (Human)

CAT: 0247-OKCD01139-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD01139-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Farnesyl Diphosphate Synthase (FDPS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Farnesyl Diphosphate Synthase (FDPS) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Farnesyl diphosphate synthase
Gene Aliases
(2E,6E) -farnesyl diphosphate synthase; Dimethylallyltranstransferase; Farnesyl diphosphate synthase; farnesyl pyrophosphate synthase; farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase; FPP synthase; FPP synthetase; FPPS; FPS; Geranyltranstransferase; POROK9.
Gene ID
2224
Accession Number
NP_001129293
Reactivity
Homo sapiens|Human
Target
Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Farnesyl Diphosphate Synthase (FDPS) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Farnesyl Diphosphate Synthase (FDPS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Farnesyl Diphosphate Synthase (FDPS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Farnesyl Diphosphate Synthase (FDPS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
0.312-20ng/mL
Recovery
Matrix Recovery Range (%) Average (%) serum (n=5) 98-105 102 EDTA plasma (n=5) 80-94 89 heparin plasma (n=5) 80-101 96 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 98-105 102 serum (n=5) 98-105 102 EDTA plasma (n=5) 80-94 89 EDTA plasma (n=5) 80-94 89 heparin plasma (n=5) 80-101 96 heparin plasma (n=5) 80-101 96
Sensitivity
< 28.3 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-FDPS Microplate 96 Wells (12 x 8 Well Strips) FDPS Lyophilized Standard 2 x 4 ng 100X Biotinylated FDPS Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-FDPS Microplate 96 Wells (12 x 8 Well Strips) Anti-FDPS Microplate 96 Wells (12 x 8 Well Strips) FDPS Lyophilized Standard 2 x 4 ng FDPS Lyophilized Standard 2 x 4 ng 100X Biotinylated FDPS Detector Antibody 120 uL 100X Biotinylated FDPS Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBWA3
Specificity
This assay has high sensitivity and excellent specificity for detection of Farnesyl Diphosphate Synthase (FDPS) . No significant cross-reactivity or interference between Farnesyl Diphosphate Synthase (FDPS) and analogues was observed.
Protein Length
419
NCBI Gene Symbol
FDPS
Protein Name
Farnesyl pyrophosphate synthase
Gene Name URL
FDPS
Nucleotide Accession Number
NM_001135821.1

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